| 摘要: |
| 为明确eIF5B-1基因在拟南芥种子萌发过程中的生理作用。以拟南芥翻译起始因子的T-DNA插入突变体eif5b-1为材料。测定突变体eif5b-1种子的萌发速率，同时利用多聚核糖体谱和qRT-PCR的研究方法，对拟南芥野生型（WT）和eif5b-1突变体的萌发过程进行了研究。结果表明：1）eif5b-1突变体种子的萌发率下降；2）eif5b-1突变体种子萌发过程中多聚核糖体的形成受到影响；3）eif5b-1突变体中ABI1、ABI3、ABI4、ABI5及DOG1基因的转录受到影响；4）eif5b-1突变体选择性地影响ABI4和DOG1基因的翻译效率。 |
| 关键词: 拟南芥 翻译起始因子 种子 萌发 翻译 脱落酸 |
| DOI：10.11841/j.issn.1007-4333.2018.01.02 |
| 投稿时间：2016-11-09 |
| 基金项目:国家自然科学基金项目（31571747）；转基因重大专项（2016ZX08002-002） |
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| Molecular regulatory mechanism of Arabidopsis translation initiation factor eIF5B-1 on seeds germination |
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KOU Xiaoxia, ZHANG Liyuan, ZHAO Yue, TIAN Xuejun, GUO Weilong, XIN Mingming, PENG Huiru
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| (College of Agronomy and Biotechnology/State Key Laboratory forAgrobiotechnology/Key Laboratory of Crop Heterosis and Utilization(Ministry of Education), China Agricultural University, Beijing 100193, China) |
| Abstract: |
| A novel T-DNA insertion mutant of Arabidopsis translation initiation factor, eif5b-1, was identified to study the regulation of seed germination in Arabidopsis. Compared to WT, the germination rate of eif5b-1 mutant seeds was investigated. Using polysome profiling and qRT-PCR, the germination process of WT and eif5b-1 mutant were analyzed. The results showed that:1) The germination rate of eif5b-1 mutant was decreased;2) The polysome profiling was abnormal in eif5b-1 mutant;3) The transcription levels of ABI1, ABI3, ABI4, ABI5 and DOG1 were changed in eif5b-1 mutant;4) The translation efficiency of ABI4 and DOG1 were selectively changed in eif5b-1 mutant. |
| Key words: Arabidopsis translation initiation factor seeds germination translation ABA |
