| 摘要: |
| 为获得梅花鹿Ghrelin cDNA全序列，以梅花鹿皱胃黏膜上皮组织提取的总RNA为模板，通过RT-PCR和RACE法克隆了梅花鹿皱胃中Ghrelin基因cDNA的全序列。结果表明梅花鹿Ghrelin cDNA序列全长为539 bp，其中5'非翻译区（5'UTR）为46 bp，3'UTR为128 bp，开放阅读框（ORF）为351 bp，该ORF编码116个氨基酸残基。将梅花鹿Ghrelin基因的cDNA与人和其他动物的Ghrelin相比，发现：梅花鹿Ghrelin与驯鹿、山羊、绵羊和牛的同源性达90.4%~99.1%；与恒河猴、人、猪、犬的同源性达76.6%~66.9%；与鸡和野鸽的同源性分别为36.4%和35.4%。研究表明Ghrelin的结构具有明显的种属特异性，因此Ghrelin在反刍动物体内可能有着重要的生理功能。 |
| 关键词: 梅花鹿 Ghrelin cDNA克隆 同源性分析 |
| DOI：10.11841/j.issn.1007-4333.2017.12.11 |
| 投稿时间：2016-10-26 |
| 基金项目:内蒙古自然科学基金资助项目（2013MS0408） |
|
| Full-length cDNA sequence cloning and analysis of Ghrelin in Cervus nippon |
|
ZHANG Man, JIN Xin, TIAN Qiaozhen, LIU Jiao, WANG Yunhe, YANG Yinfeng
|
| (College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China) |
| Abstract: |
| In order to obtain the full-length cDNA of Ghrelin in Cervus nippon,RT-PCR and RACE methods were used by using total RNA of abomasus tissue in C.nippon as template.The results of sequence analysis revealed a 539 bp length cDNA containing 46 bp 5'-untranslated region (5'UTR),128 bp 3'-untranslated region(3'UTR)and 351 bp open reading frame (ORF) encoding 116 amino acids.The cDNA sequence alignments of C.nippon Ghrelin gene with human and other animals showed that the cDNA sequence homology of C.nippon Ghrelin was 90.4%-99.1% to reindeer,goat,sheep and cattle,66.9%-76.6% with rhesus monkey,human,pig and dog,only 36.4% with chicken and C.livia.These results indicated that the structure of Ghrelin displayed an obvious varietal specificity,suggesting that Ghrelin might play an important physiological function role in ruminants. |
| Key words: Cervus nippon Ghrelin cDNA clone homology analysis |
