| 摘要: |
| 为探讨β-胡萝卜素不同添加方式（预防型和治疗型）对脂多糖（LPS）刺激的RAW264.7细胞的细胞活力、活性氧（ROS）、炎性因子以及NF-κB通路的影响，利用MTT法检测细胞活力确定β-胡萝卜素的最佳添加浓度和时间，流式细胞仪检测ROS的百分含量，荧光定量PCR检测炎性因子IL-1β、IL-6和TNF-α mRNA相对表达量；Western Blot测NF-κB p65蛋白的表达量分析β-胡萝卜素对NF-κB通路的影响。结果表明：对于LPS刺激的RAW264.7细胞，治疗型和预防型添加β-胡萝卜素均可以提高细胞活力，且在治疗型添加模型中，可极显著提高（P<0.01）；治疗型和预防型添加β-胡萝卜素均可以降低ROS百分含量、炎性因子mRNA相对表达量和NF-κB p65蛋白表达量。综上所述，不同添加方式的β-胡萝卜素对LPS刺激的巨噬细胞的细胞活力均有提升作用，对ROS和炎性因子均有抑制作用，且这种抑制作用可能与NF-κB通路有关。 |
| 关键词: β-胡萝卜素 RAW264.7细胞 细胞活力 活性氧 炎性因子 |
| DOI：10.11841/j.issn.1007-4333.2017.11.13 |
| 投稿时间：2016-11-25 |
| 基金项目:国家自然科学基金面上项目（31672511）。 |
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| Effects of different β-carotene adding models on the inflammation of LPS-induced RAW264.7 cells |
|
ZHANG Xiaoyin, WU Min, ZHANG Shanshan, JI Yubin, HUANG Yusheng, ZHENG Xin
|
| (College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China) |
| Abstract: |
| The aim of this study was to investigate the effects of β-carotene treatment model and prevention model on cell viability,ROS,inflammatory factors and NF-κ B p65 protein in LPS-induced RAW264.7 cells.To determine the most suitable concentration and time of β-carotene,the cell viability in two models was measured by MTT.The percentage of ROS was detected by flow cytometry analysis,and the mRNA relative expressions of IL-1 β, IL-6 and TNF-α were measured by fluorescence quantitative PCR.The protein relative expressions of NF-κ B p65 were measured by Western Blot to research the effect of β-carotene on NF-κ B signaling pathway.The results showed that: In LPS-induced RAW264.7 cells,the cell viabilities of treatment model and prevention model were higher and the difference was very significant in treatment model (P<0.01);The percentage of ROS,expression of inflammatory factors and protein NF-κ B p65 were lower than LPS-induced group.In summary, β-carotene promoted the cell viability of LPS-induced RAW264.7 cells and inhibited the percentage of ROS and expression of inflammatory factors,indicating the correlation of the inhibition effect with NF-κ B pathway. |
| Key words: β-carotene RAW264.7 cell cell viability ROS inflammatory factor |
