| 摘要: |
| 研究牛乳蛋白过敏儿童血清中对4种主要乳蛋白过敏原的抗体特异性情况。采用间接酶联免疫吸附法测定乳过敏儿童血清中4种乳蛋白过敏原特异性IgE抗体。结果显示，乳过敏儿童血清中α-乳白蛋白和β-乳球蛋白的特异性IgE比例较高，特异性IgE阳性率分别可达44.3%和39.3%，其次α-酪蛋白和β-酪蛋白特异性IgE阳性率为31.2%和36.1%。研究结果表明，乳过敏儿童对4种乳蛋白过敏原都有一定比例的特异性IgE抗体，其中α-LA和β-LG的阳性率较高，是乳蛋白过敏儿童的主要过敏原蛋白，大部分乳过敏儿童不止对1种乳蛋白过敏。 |
| 关键词: 牛乳蛋白 过敏儿童 特异性IgE 过敏原 |
| DOI：10.11841/j.issn.1007-4333.2017.09.05 |
| 投稿时间：2016-07-13 |
| 基金项目:国家自然科学基金（31171715） |
|
| Detection and analysis of binding activities of IgE antibodies from child patients allergic to cow's milk protein |
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SHI Jing1, ZHOU Yadi2, LUO Yongkang1, JIANG Mizuo3
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| (1.College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China;2.Beijing Shuangwa Dairy Co., Ltd., Beijing 100083, China;3.Children's Hospital Affiliated with Zhejiang University, Hangzhou 310001, China) |
| Abstract: |
| Aiming to investigate 4 kinds of milk protein allergen specific IgE in serum of children with cow milk protein allergy.Specific IgE antibodies for 4 kinds of milk protein allergens were detected in milk-allergic children by enzyme-linked immunosorbent assay (ELISA).The results demonstrated that:α-LA- and β-LG-specific IgE in milk allergy children serum were maintained in a high level,and the positive rate reached 44.3% and 39.3%;The positive rates of α-CN- and β-CN-specific IgE were respectively 31.2% and 361%.These results suggested that milk-allergic children had a certain proportion of specific IgE of 4 kinds of milk protein allergen. α-LA and β-LG were probably the main allergens of milk allergy children with higher positive rate.Most of milk-allergic children were allergic to more than one kind of milk protein allergen. |
| Key words: cow milk protein allergic children specific IgE allergen |
