| 摘要: |
| 为探明骆驼斯氏副柔线虫雌虫和雄虫的转录组差异，挖掘出与性别决定相关的功能基因，采用Illumina HiSeqTM2000分别对其雌虫和雄虫样本进行转录组测序，构建cDNA文库，通过建库质量评估和组装效率评估后对其进行功能注释及相关生物信息学分析。结果显示，比较雌虫和雄虫的转录本，获得6 430个差异表达基因，其中2 131个基因上调；4 299个基因下调。对差异表达基因进行GO功能分类，有341 328和714个Unigenes分别注释到生物学过程、细胞组成和分子功能三大类41个分支中。KEGG数据库分析，有1 116个差异表达基因参与到198条KEGG通路中，显著富集在机体免疫调节，卵母细胞成熟等通路。功能聚类分析中，雌虫样本在生殖发育、产卵、生殖行为中高表达；雄虫在主要精子蛋白、丝氨酸/苏氨酸特异性蛋白磷酸酶中高表达。此外，在雄虫和雌虫中筛选出Tab-3，Tab-5、Fem-1和Mog-3等10种线虫性别决定相关基因，以及8种雄性性别特异表达基因和4种雌性性别特异表达基因，这些基因在虫体性别决定和性别特异性表达中发挥重要作用。本研究利用生物信息学技术预测出雌虫和雄虫差异表达基因在功能分类和代谢通路等方面的生物学特征，挖掘出性别决定基因和性别特异性表达相关基因，为进一步开展斯氏副柔线虫的功能基因组学研究、药物靶点预测和疫苗候选基因筛选提供有价值的数据资源。 |
| 关键词: 斯氏副柔线虫 转录组 差异表达基因 生物信息学分析 |
| DOI：10.11841/j.issn.1007-4333.2017.07.008 |
| 投稿时间：2016-06-08 |
| 基金项目:国家自然科学基金资助项目（31260603） |
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| Comparative transcriptome analysis of female and male adults of Parabronema skrjabini in camel |
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FENG Chenchen, WANG Wenlong, HONG Mei, LIU Ning, Huhebateer
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| (College of Veterinary Medicine, Inner Mongolia Agricultural University/Key Laboratory of Clinical Diagnosis and Treatment Technology in Animal Disease, Ministry of Agriculture, Hohhot 010018, China) |
| Abstract: |
| Aiming to find sex-determining genes,transcriptome profile of female and male adults of Parabronema skrjabini in camel were investigated.Samples of female and male were analyzed using Illumina HiSeq2000TM RNA sequencing platform and their transcriptome libraries were constructed.After quality filtering and de novo assembling,functional annotation and bioinformatics analysis were carried out.The results showed that:A total of 6 430 differentially expressed genes between female and male were obtained,of which 2 131 and 4 299 of genes were up-and down-regulated,respectively.And 341 328 and 714 differentially expressed genes were annotated into biological process,cellular component and molecular function and 41 functional categories.KEGG analysis showed that 1 116 differentially expressed genes were assigned to 198 KEGG pathways,and clustered significantly in immuno regulation,oocyte maturation and so on.Functional cluster analysis indicted that reproductive development,oviposition and reproductive behavior were high enriched in female adult stage,whilst the sperm main protein,serine/threonine specific protein phosphorylas etc.were high enriched in male adults stage.Moreover,we identified ten the significant sex-determining genes,such as Tab-3,Tab-5 and Fem-1,eight gender-specific expression genes of male adult and four gender-specific expression genes of female adult.These genes were necessary to decide nematode gender.In this study,the biological characteristics of differentially expressed genes were predicted by bioinformatics,which involved in functional genes and metabolic pathways.This study provides a foundation for the further whole genome sequence analysis,drug target prediction and vaccine candidate gene screening. |
| Key words: Parabronemas krjabini transcriptome differentially expressed genes bioinformatics analysis |
