| 本文已被：浏览 676次 下载 287次 |
码上扫一扫！ |
| 日粮中添加苜蓿黄酮对奶牛血液生化指标、抗氧化性能和免疫的影响 |
|
占今舜1,2, 苏效双1, 刘明美1,3, 詹康1, 张春刚1,4, 赵国琦1
|
|
|
| (1.扬州大学 动物科学技术学院, 江苏 扬州 225009;2.江西省农业科学院 畜牧兽医研究所, 南昌 330200;3.江苏联合职业技术学院 淮安生物工程分院, 江苏 淮安 223200;4.上海光明荷斯坦牧业有限公司, 上海 200443) |
|
| 摘要: |
| 为研究苜蓿黄酮对奶牛血液生化指标、抗氧化性能和免疫的影响，选取4头装有瘘管的头胎荷斯坦奶牛，试验采用4×4拉丁方设计，每组奶牛饲喂混合日粮中分别添加0 g（Ⅰ组）、20 g（Ⅱ组）、60 g（Ⅲ组） 和100 g（Ⅳ组）的苜蓿黄酮。试验期为4期，每期24 d。结果显示：1）血清的总蛋白含量随苜蓿黄酮添加剂量的升高呈二次曲线变化，出现先下降后升高（P＜0.05）趋势，但其他指标没有影响；2）苜蓿黄酮组血液中的血小板数量（P＜0.05）和血小板压积（0.05<P≤0.10）均高于对照组，但随着苜蓿黄酮添加剂量的升高而下降；中性粒细胞比例随苜蓿黄酮添加剂量的升高呈线性升高（P＜0.05），而淋巴细胞比例则呈降低的趋势（0.05<P≤0.10）；3）谷胱甘肽过氧化物酶（GSH-PX）活性随苜蓿黄酮添加剂量的升高呈线性升高（P≤0.05），而丙二醛（MDA）含量则呈现降低的趋势（0.05<P≤0.10），过氧化氢酶（CAT）活性随苜蓿黄酮添加剂量的升高呈先升高后降低（P＜0.05）；4）血清中IgG含量（0.05<P≤0.10）和淋巴细胞Fas基因相对表达（P＜0.05）随着苜蓿黄酮添加剂量的升高呈先升高后下降的趋势。以上结果表明，苜蓿黄酮能够提高机体的抗氧化性能和调节机体免疫。 |
| 关键词: 奶牛 苜蓿黄酮 血液生化指标 抗氧化性能 免疫力 |
| DOI：10.11841/j.issn.1007-4333.2017.05.008 |
| 投稿时间：2016-05-04 |
| 基金项目:江苏省高校优势学科建设工程项目（PAPD）；上海市科委国内科技合作项目（14395810100）；江苏省高校研究生科研创新计划项目（KYZZ_0367） |
|
| Effects of diet added alfalfa flavonoids on blood biochemical indexes, oxidation resistance and immunity of dairy cow |
|
ZHAN Jinshun1,2, SU Xiaoshuang1, LIU Mingmei1,3, ZHAN Kang1, ZHANG Chungang1,4, ZHAO Guoqi1
|
| (1.College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China;2.Institute of Animal Husbandry and Veterinary, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China;3.Jiangsu Joint Institute of Technology of Profession of Huai’an Bio-Engineering Branch, Huai’an 223200, China;4.Shanghai Bright Holstan Co., Ltd, Shanghai 200443, China) |
| Abstract: |
| The objective of this study was to examine the effects of flavonoids from alfalfa on blood biochemical indexes,oxidation resistance and immunity of dairy cows.Four primiparous Holstein cows fitted with ruminal cannulas were selected.4×4 Latin square design was used in the experiment and the cows were fed total mixed ration added 0,20,60 or 100 g alfalfa flavonoids at different periods,respectively.The experiment had four experiment periods and each period contained 24 days.The results showed:1) The total protein content of serum appeared change of quadratic curve,and was decreased at first and then increased with increasing doses of alfalfa flavonoids extract (AFE) (P＜0.05).The other indexes were not affected by AFE;2) Platelet counts (P＜0.05) and thrombocytocrit (0.05<P≤0.10) were higher than that of control group,and showed a tendency of decrease with increasing the dose of AFE.The proportion of neutrophil was increased linearly (P＜0.05),whereas the proportion of lymphocyte displayed a decline trend (0.05<P≤0.10) with the increasing level of AFE;3) The glutathione peroxidase activity was increased linearly (P≤ 0.05),whereas the methane dicarboxylic aldehyde concentration had a tendency of decrease (0.05<P≤ 0.10) with increasing the level of AFE.The catalase activity was increased at first and then decreased with increasing the dose of AFE (P＜0.05);4) The Ig G concentration of serum (0.05<P≤0.10) and the relative expression of Fas gene in lymphocyte (P＜0.05) was increased first and then decreased with the increasing dose of AFE.In conclusion,AFE could improve oxidation resistance and regulate the immunity of dairy cow. |
| Key words: dairy cow alfalfa flavonoids blood biochemical indexes oxidation resistance immunity |
