| 摘要: |
| 为研究饲料中添加不同比例N-HMM-Ca对泌乳期奶牛血清生化指标的影响，试验选用胎次、产奶量和泌乳天数相近身体健康的荷斯坦奶牛30头，完全随机区组分为5组，每组6头。对照组饲喂基础日粮；试验组分别饲喂在基础日粮中以干物质基础添加0.15%、0.30%、0.75%和1.50%的N-HMM-Ca，预试期14 d，正试期42 d。结果表明：1）泌乳期奶牛日粮中添加0.75% N-HMM-Ca使血清中谷丙转氨酶（ALT）和谷草转氨酶（AST）浓度显著升高。2）在第14天时，1.50%组甘油三酯（TG）浓度显著高于其他各处理组（P<0.01）。血清中高密度脂蛋白（HDL）水平有所提高，但差异不显著（P>0.05）；随着N-HMM-Ca添加量的增加血清中非酯化脂肪酸（NEFA）的浓度逐渐降低（P<0.05）；对β-羟丁酸（β-OHB）来说，随着日粮中N-HMM-Ca添加量的增加浓度逐渐升高（P<0.05）。3）0.15%、0.30%和0.75%组血清IgA浓度呈现增加的趋势，而且在第42天时尤为显著（P<0.01）。4）在第28天时，泌乳期奶牛日粮中分别添加0.15%和0.30%的N-HMM-Ca使过氧化氢酶（CAT）浓度显著高于其他各处理组（P<0.01）。综上所述，泌乳期奶牛日粮中添加0.15%的N-HMM-Ca有利于改善机体健康。 |
| 关键词: 荷斯坦奶牛 N-HMM-Ca 血清酶 免疫指标 抗氧化指标 |
| DOI：10.11841/j.issn.1007-4333.2017.03.12 |
| 投稿时间：2016-03-21 |
| 基金项目:江苏省高校优势学科建设工程项目（PAPD）；上海市科学技术委员会国内科技合作项目（14395810100） |
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| Effects of N-hydroxymethyl methionine calcium in dietary on serum biochemical parameters of lactating dairy cows |
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SUI Yannan, MA Tingting, CHEN Zhiyuan, YU Tongshui, GONG Xiaoxiao, ZHAN Kang, ZHAO Guoqi
|
| (College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China) |
| Abstract: |
| To study the effects of different N-hydroxymethyl methionine calcium (N-HMM-Ca) supplementation levels on serum's biochemical indexes of lactating dairy cows.Thirty lactating Holstein dairy cows,which have similar parity,milk yield and lactating stage,were randomly divided into 5 groups with 6 cows in each group.The control group was fed with basal diet,the test groups were fed with 0.15%,0.30%,0.75% and 1.50% levels of N-HMM-Ca of basal diet (on DM basis),respectively.The experiment lasted up to 56 d,including 14 d of adaptation period and 42 d of experimental period.The results showed that:1) Supplemented 0.75% dosage of N-HMM-Ca on the diet improved the serum ALT and AST concentrations;2) On the 14th day of experimental period,after supplemented 1.50% N-HMM-Ca the triglyceride (TG) concentration was significantly increased (P<0.01).Serum high-density lipoprotein (HDL) and β-hydroxy butyric acid (β-OHB) concentration were increased with N-HMM-Ca supplementation,nevertheless,serum non-esterified fatty acid (NEFA) was decreased linear;3) Serum IgA concentration was significantly increased in 0.15% and 0.30% groups on 42th day (P<0.01);4) On 28th day,serum catalase (CAT) concentration was significant higher in 0.15% and 0.30% level than in other treatments (P<0.01).In conclusion,0.15% N-HMM-Ca supplementation in the diet was beneficial to improve the health of dairy cows. |
| Key words: cows N-HMM-Ca biochemical serum enzyme immune index antioxidant indices |
