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| 产后奶牛子宫内致病菌的分离、毒力基因检测及耐药性分析 |
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贾知锋1, 王纯洁1, 敖日格乐2, 高瑞娟1, 徐进1, 高思明1, 包胡斯楞2, 阿日查2, 郭雪梅2
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| (1.内蒙古农业大学 兽医学院, 呼和浩特 010018;2.内蒙古农业大学 动物科技学院, 呼和浩特 010018) |
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| 摘要: |
| 为检测产后奶牛子宫内膜炎致病菌的毒力基因和耐药性，在生产3周后采用棉拭子法对65头确诊的临床型子宫内膜炎荷斯坦奶牛进行子宫黏液的采集；分别用阴门灌注大鼠子宫试验、PCR方法以及药敏纸片法来检验分离株的致病性。结果表明，灌注混合菌（1∶1，1.0×109 cfu/mL）72 h后剖解大鼠子宫发现子宫充血，肿大，增厚，子宫内部有蓄脓现象。然而，Escherichia coli和Staphylococcus aureus从患病牛中最终检出率分别为58.69%、62.75%；并对27株致病性E.coli系统发育组ChuA、yjaA基因检出率达到（13/27，48.15%），且E.coli和S.aureus 分离株中均含有毒力基因KpSMII和TSST-1；随机抽取10头患病牛且含毒力基因最多的分离株进行药敏试验且均耐受于青霉素、多西环素、多粘菌素B、阿莫西林和四环素等抗生素。由此可见，以上抗生素再继续应用将会导致E.coli和S.aureus耐药程度持续增加。 |
| 关键词: 奶牛 子宫内膜炎 大肠杆菌 金黄色葡萄球菌 毒力基因 药敏试验 |
| DOI：10.11841/j.issn.1007-4333.2017.02.008 |
| 投稿时间：2016-02-02 |
| 基金项目:国家自然科学基金项目（31260590）；国家科技支撑项目（2015BAD29B06） |
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| Separation,detection of virulence genes and drug-resistance analysis of pathogenic bacteria from the uterine of postpartum dairy cows |
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JIA Zhifeng1, WANG Chunjie1, Aorigele2, GAO Ruijuan1, XU Jin1, GAO Siming1, Baohusileng2, Aricha2, GUO Xuemei2
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| (1.College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China;2.College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China) |
| Abstract: |
| The objective of this study was to investigate the relationship between bacteria species-specific virulence facts (VFs) and drug resistance present in the uterus of postpartum dairy cows.Purulent or mucopurulent uterine exudates in the vagina occurring after the third week of lactation from 65 postpartum cows were collected by using uterine swabs.Rat's uterus test,PCR,and susceptibility paper method were used to detect pathogenicity.The results showed that this rat's uterine perfused with mixed bacteria(1:1,1.0×109 cfu/mL) displayed uterine congestion,swelling,thickening of the uterine internal pyometra phenomenons after 72 hours.However,final detection of Escherichia coli and Staphylococcus aureus in diseased cows were 58.69% and 62.75,respectively.The dectection rate of ChuA and yjaA in pathogenic E.coli reached 48.15% and E.coli and S.aureus isolated contained virulence KpsMII and Tsst-1 respectively.In randomly selected ten sick cows and most isolated virulence genes isolates were tolerance to penicillin,doxycycline,polymyxin B,amoxicillin,tetracycline,and other antibiotics.In conclusion,continuing application of these antibiotics will result in increasing of E.coli and S.aureus resistance level. |
| Key words: cow endometritis Escherichia coli Staphylococcus aureus virulence genes antibiotic resistance |
