| 摘要: |
| 为探讨8种微量添加物（维生素B1、核黄素、烟酰胺、腺嘌呤、D-泛酸钙、叶酸、钴胺素以及甘氨酸与腺嘌呤混合物）对蛹虫草菌发酵液虫草素产量的影响，筛选提高虫草素产量的最佳方法，将不同质量浓度的微量添加物单独加入蛹虫草液体发酵培养基中，以HPLC法测定发酵液的虫草素产量。结果表明：甘氨酸和腺嘌呤的混合物、腺嘌呤、核黄素、D-泛酸钙均能显著促进虫草素的生物合成，适宜添加质量浓度为：甘氨酸14 g/L和腺嘌呤2 g/L的混合物、腺嘌呤2 g/L、核黄素1 g/L、D-泛酸钙2 g/L；维生素B1和钴胺素均能促进虫草素的生物合成，适宜添加质量浓度均为2和2 g/L；而烟酰胺和叶酸均抑制虫草素的生物合成。其中，添加甘氨酸14 g/L和腺嘌呤2 g/L的混合物对发酵液虫草素产量的提高作用最显著，比对照提高了103%。当甘氨酸与腺嘌呤的质量浓度比为7∶1时，两者通过协同互补过程促进虫草素合成的作用最为显著；此外，本研究认为甘氨酸和腺嘌呤分别由从头合成和补救合成途径共同促进了虫草素的生物合成。 |
| 关键词: 蛹虫草 微量添加物 液体发酵 虫草素 |
| DOI：10.11841/j.issn.1007-4333.2017.01.09 |
| 投稿时间：2016-01-20 |
| 基金项目:甘肃省科学院青年科技创新基金项目（2014QN-07）；甘肃省科学院青年科技创新基金项目（2013QN-05） |
|
| Effects of different additives on cordycepin production by liquid fermentation using Cordyceps militaris mycelia |
|
QIN Peng, WANG long, LU Dengxue, ZHAO Yuhui, HAN Rongbing
|
| (Institute of Biology, Gansu Academy of Sciences, Lanzhou 730000, China) |
| Abstract: |
| In order to select additive from 8 kinds of additives and its optimal concentration,different concentrations of vitamin B1,riboflavin,nicotinamide,adenine,D-calcium pantothenate,folic acid,cobalamin,mixture of glycine and adenine were added to liquid fermentation broth,and the effects of these additives on cordycepin production by Cordyceps militaris mycelia were detected by HPLC.The results showed that four additives promoted cordycepin production significantly.These additives were mixthure of glycine and adenine (14 and 2 g/L),adenine (2 g/L),riboflavin (1 g/L),D-calcium pantothenate (2 g/L).Vitamin B1 and cobalamin were less effective additives while nicotinamide,and folic acid inhibited cordycepin production.The mixture of glycine and adenine (14 g/L and 2 g/L,respectively) was the best of 8 kinds of additives where the production of cordycepin reached 300.868 μg/mL.Synergism effect was strongest of all ratios when the concentration ratio between glycine and adenine was 7:1.Glycine and adenine promoted meterials production of biosynthesis of cordycepin through denovo and salvage pathway,respectively. |
| Key words: Cordyceps militaris trace additives liquid fermentation cordycepin |
