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| 地衣芽孢杆菌H2对坏死性肠炎肉鸡生长性能及肠道菌群的影响 |
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周梦佳1,2, 曾东1,2, 倪学勤1,2, 涂腾3, 王鹤松1,2
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| (1.四川农业大学 动物医学院/动物微生态研究中心, 成都 611130;2.动物疫病与人类健康四川省重点实验室, 成都 611130;3.四川农业大学 动物科技学院/动物遗传育种研究所, 成都 611130) |
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| 摘要: |
| 为研究地衣芽孢杆菌H2对坏死性肠炎肉鸡生长性能及肠道菌群的影响，选取240只1日龄科宝-500肉鸡，随机分为Ⅰ组（空白对照组）、Ⅱ组（造模组）、Ⅲ组（球虫+鱼粉组）和Ⅳ组（地衣芽孢杆菌防治组）。Ⅱ组、Ⅲ组和Ⅳ组从14日龄起饲喂添加30%鱼粉的日粮并于15日龄灌喂球虫，Ⅱ组和Ⅳ组18~20日龄连续灌喂产气荚膜梭菌（2.2×108 cfu/mL）1 mL，其余各组灌喂同等剂量的生理盐水，试验期总共为22 d。结果表明：坏死性肠炎的发生会显著降低肉鸡体增重升高料重比（P<0.05），地衣芽孢杆菌的添加能提高坏死性肠炎肉鸡体增重。DGGE图谱聚类结果显示，肉鸡回肠内容物菌群主要聚为两簇，Ⅳ组和Ⅰ组聚为一簇，Ⅱ组和Ⅲ组聚为一簇，相似性为0.70。此外，与Ⅱ组相比，Ⅰ组和Ⅳ组回肠内容物中乳杆菌属数量显著增加（P<0.05），Ⅰ组肠杆菌科数量显著降低（P<0.05），Ⅳ组肠杆菌科数量降低且与Ⅰ组无显著差异（P>0.05）。综上所述，在日粮中添加地衣芽孢杆菌能改善坏死性肠炎肉鸡生长性能，调节由坏死性肠炎导致的菌群失调。 |
| 关键词: 地衣芽孢杆菌 肉鸡 坏死性肠炎 生长性能 肠道菌群 |
| DOI：10.11841/j.issn.1007-4333.2017.01.07 |
| 投稿时间：2016-02-26 |
| 基金项目:四川省科技厅国际合作项目（2013HH0055） |
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| Effects of Bacillus licheniformis H2 on the growth performance and gut microflora of broiler chickens infected with necrotic enteritis |
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ZHOU Mengjia1,2, ZENG Dong1,2, NI Xueqin1,2, TU Teng3, WANG Hesong1,2
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| (1.Animal Microecology Institute, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China;2.Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, China;3.Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China) |
| Abstract: |
| To investigate the effects of Bacillus licheniformis on the growth performance and gut microflora in broiler chickens infected with necrotic enteritis (NE),a total of 240 one-day-old broilers were randomly divided into four groups:GroupⅠ(negative control),group Ⅱ(NE experimental model),group Ⅲ(chickens fed with diet containing 30% fishmeal and were infected with coccidiosis),group Ⅳ(NE group supplied with feed containing B.licheniformis).From day 14 onwards,chickens form groupsⅡ,Ⅲ and Ⅳ were given a diet containing 30% fishmeal and were given with coccidian on day 15.All birds in groupⅡand Ⅳ were fed with C.perfringens(2.2×108 CFU/mL) through oral gavage from day 18 to day 20.The trial lasted for 22 days.The results showed that:Necrotic enteritis reduced the bodyweight gain significantly and impaired feed weight ratio (P<0.05);Dietary supplementation with B.licheniformis increased the bodyweight gain of broilers;Clustering analysis of ileum microflora by DGGE indicated two clusters were formed,and the similarity reached 0.70;Moreover,compared with group Ⅱ,the amount of Lactobacillus spp.was significantly higher in group Ⅰand Ⅳ(P<0.05),and the amount of Enterobacteriaceae family was significantly lower in group Ⅰ(P<0.05) and Ⅳ and no significant difference was found compared with groupⅠand Ⅳ(P>0.05).Therefore,dietary supplementation with B.licheniformis could improve the growth performance of broilers and adjust the imbalance of flora caused by NE. |
| Key words: Bacillus licheniformis broiler necrotic enteritis growth performance gut microflora |
