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| 牛种布鲁氏菌RpoE2蛋白部分生物学特性研究 |
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董浩1,2, 董礼华3, 彭小薇1,4, 王宁1, 曲萍2, 孙雨2, 赵柏林2, 胡冬梅2, 石慧2, 王晓英2, 宋晓晖2, 吴清民1
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| (1.中国农业大学 动物医学院, 北京 100193;2.中国动物疫病预防控制中心, 北京 102600;3.乳山市夏村镇畜牧兽医工作站, 山东 乳山 264500;4.中国兽医药品监察所, 北京 100081) |
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| 摘要: |
| 为探讨牛种布鲁氏菌sigma因子RpoE2的功能，采用酵母双杂交技术对RpoE2蛋白与anti-sigma因子之间的互作进行研究；通过环境应激试验评价了rpoE2缺失株对强氧化环境、酸性pH、杀菌性阳离子多肽、铁缺乏环境的耐受能力；通过比较亲本菌株和缺失株在巨噬细胞感染模型和小鼠感染模型中的生存能力，研究了rpoE2基因是否与牛种布鲁氏菌毒力相关。结果显示：牛种布鲁氏菌的RpoE2蛋白可以与anti-sigma因子相互作用，牛种布鲁氏菌rpoE2基因缺失后，对各种应激环境的耐受能力均无影响，并且巨噬细胞和小鼠感染模型中毒力均不降低。牛种布鲁氏菌的RpoE2蛋白是一个ECF16家族的sigma因子，并且其与布鲁氏菌的毒力无关。 |
| 关键词: 牛种布鲁氏菌 ECF16家族sigma因子 毒力 环境应激 |
| DOI：10.11841/j.issn.1007-4333.2017.01.05 |
| 投稿时间：2016-01-11 |
| 基金项目:国家973项目（2010CB530202）；国家自然科学基金资助项目（30871882） |
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| Study on biological characteristic of RpoE2 in Brucella abortus |
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DONG Hao1,2, DONG Lihua3, PENG Xiaowei1,4, WANG Ning1, QU Ping2, SUN Yu2, ZHAO Bolin2, HU Dongmei2, SHI Hui2, WANG Xiaoying2, SONG Xiaohui2, WU Qingmin1
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| (1.College of Veterinary Medicine, China Agriculture University, Beijing 100193, China;2.China Animal Disease Control Centre, Beijing 102600, China;3.Animal Husbandry and Veterinary Station of Xiacun County, Rushan 264500, China;4.China Institute of Veterinary Drug Control, Beijing 100081, China) |
| Abstract: |
| To investigate the function of RpoE2 in Brucella abortus,yeast two-hybird system was used to verify the interaction between RpoE2 and anti-sigma factor.Stress response assays were also performed to evaluate if the rpoE2 mutant tolerance to various stresses (oxidative stress,detergents,antimicrobial peptides,extreme pH,and iron limited).The survival ratios of rpoE2 mutant and WT in both J774A.1 cell and mouse infection model were also investigated.The results showed that RpoE2 interacted with anti-sigma factor,and the rpoE2 null mutant was not defective for the resistance to various stresses.Moreover,the survival ratio of all Brucella wild type strain and its mutant was almost the same in both macrophage and mice infection models.All these data demonstrated that RpoE2 was an ECF 16 sigma factor,and not involved in bacterial virulence in B.abortus. |
| Key words: Brucella abortus ECF16 sigma factor virulence stress response |
