| 摘要: |
| 为探讨鸡甲基转移酶样蛋白5（Methyltransferase like protein 5，METTL5）基因的序列和结构，采用RT-PCR方法克隆该基因，并用生物信息学方法分析该基因的特征；利用定量PCR（qPCR）方法检测METTL5基因的组织表达模式和母鸡叶酸缺乏对仔鸡腹脂中该基因表达的影响。结果表明，鸡METTL5基因的cDNA序列长度为702 bp（Genbank accession：KU351684），编码212个氨基酸；进化树分析表明鸡METTL5氨基酸序列与野鸭和鸿雁的关系较近，与非洲爪蟾的进化关系最远。鸡METTL5氨基酸序列包含保守的AdoMet_MTases superfamily结构域，为亲水蛋白；二级结构主要是α-螺旋，占44.81%；该蛋白不存在信号肽，不是分泌蛋白；亚细胞定位显示该蛋白存在于细胞质的概率为69.6%。qPCR分析表明，METTL5基因在所检测鸡的8种组织均有表达，在腹脂中表达水平最高，在脾脏中的表达水平最低；叶酸缺乏试验表明，母鸡叶酸缺乏对仔鸡腹脂组织METTL5基因表达水平有增加的趋势，但与对照组相比差异不显著（P>0.05）。 |
| 关键词: 肉鸡 METTL5 基因 腹脂 表达谱 |
| DOI：10.11841/j.issn.1007-4333.2016.12.10 |
| 投稿时间：2016-01-04 |
| 基金项目:山东省自然科学基金资助项目（ZR2013CL012）；临沂大学国家级大学生创新创业训练计划项目（201510452021）；国家自然科学基金资助项目（31372333） |
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| Molecular cloning and expression patterns analysis of a methyltransferase like protein 5 (METTL5) gene in chicken |
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XING Jin-yi, LI Jia-li, ZHU Meng-ying, SU Xin, WANG Guo-ru
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| (School of Life Science, Linyi University, Linyi 276000, China) |
| Abstract: |
| To explore sequences and structure of chicken methyltransferase like protein 5 (METTL5),the METTL5 was cloned by RT-PCR,and characterization of METTL5 was analyzed by bioinformatics methods.The expression patterns in different tissues and effects of folate deficiency in the maternal diets on METTL5 gene in offspring of broiler breeders were detected by real time RT-PCR.The results indicated that the METTL5 cDNA was 702 bp (Genbank accession:KU351684) in length,which encoded 212 amino acids.The phylogenetic tree analysis showed that the METTL5 of chicken was closely related to mallard and swan goose,but distant to African clawed frog.The METTL5 protein contained conserved AdoMet_MTases superfamily domain and was hydrophilicity protein.The secondary structure of METTL5 protein displayed that 44.81% of the METTL5 protein fold into α helix.This polypeptide contained no signal peptide in its amino acids,and was a nonsecretory protein.The residing probability of METTL5 in the cytoplasmic was 69.6%.mRNA expression levels of chicken METTL5 were detected by real time PCR in all 8 tissues investigated,and METTL5 mRNA expression level in abdominal adipose was the highest and in spleen was the lowest.Further qPCR analysis revealed that dietary folate deficiency in the maternal diets has a trend to increase the METTL5 gene expression levels in abdominal adipose of offspring of broiler breeders,but the difference was not significant compared to control group (P>0.05). |
| Key words: broiler METTL5 gene abdominal adipose expression patterns |
