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| 草莓FaSKP1-1基因的克隆与表达分析 |
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殷姗姗1, 李茂福2,3,4, 王华2,3,4, 李洋1, 张秋雷1, 金万梅2,3,4, 李天忠1
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| (1.中国农业大学 园艺学院, 北京 100193;2.北京市农林科学院 林业果树研究所, 北京 100093;3.北京市落叶果树工程技术研究中心, 北京 100093;4.农业部华北地区园艺作物生物学与种质创制重点实验室, 北京 100093) |
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| 摘要: |
| 为研究草莓中SCF复合体的功能，以栽培草莓品种‘74’为试材，采用同源克隆的方法分离出2个SKP1基因。这2个基因核苷酸序列全长均为519 bp，核苷酸序列相似性为98.46%，氨基酸序列相似性为98.84%，并具有特殊的‘GVDED’尾巴结构，分别命名为FaSKP1-1a和FaSKP1-1b（基因登录号分别为KU975057和KU975058）。RT-PCR和dCAPS分析发现FaSKP1-1a和FaSKP1-1b在草莓根、茎、叶、花托、花粉、花柱、果实中均高表达，在花瓣中表达量低。上述结果表明FaSKP1-1可能在草莓SCF复合体的形成过程中发挥重要作用。 |
| 关键词: 草莓 FaSKP1-1 基因克隆 表达分析 |
| DOI：10.11841/j.issn.1007-4333.2016.12.04 |
| 投稿时间：2016-03-02 |
| 基金项目:国家自然科学基金青年基金（31201606）；北京市农林科学院科技创新能力建设专项（KJCX20140110，KJCX20140202）；北京市农林科学院青年基金（QNJJ201507）；北京市科委重大项目（D131100000113001） |
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| Cloning and expression analysis of FaSKP1-1 genes in strawberry (Fragaria ananassa Duch.) |
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YIN Shan-shan1, LI Mao-fu2,3,4, WANG Hua2,3,4, LI Yang1, ZHANG Qiu-lei1, JIN Wan-mei2,3,4, LI Tian-zhong1
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| (1.College of Horticulfure, China Agricultural University, Beijing 100193, China;2.Institute of Forestry and Pomology, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100093, China;3.Beijing Engineering Research Center for Deciduous Fruit Trees, Key Laboratory of Biology, Beijing 100093, China;4.Genetic Improvement of Horticultural Crops(North China), Ministry of Agriculture, China, Beijing 100093, China) |
| Abstract: |
| SCF complex,thus regulating the ubiquitin degradation of organism protein.Two SKP1 family genes in cultivation strawberry, FaSKP1-1a and FaSKP1-1b,were cloned to analyze the function of SCF complex.The full-length of FaSKP1-1a and FaSKP1-1b nucleotide sequence shared the same size of 519 bp.Their nucleotide and amino acid similarity were 98.46% and 98.84%,respectively.A typical ‘GVDED’ tail structure was found in both FaSKP1-1a and FaSKP1-1b.Tissue-specific expression of FaSKP1-1a and FaSKP1-1b by RT-PCR and dCAPS showed that these two genes were highly expressed in root,leaves,stem,receptacle,pollen,style and fruit,but low in petal.These results can provide references for further research of the SCF complex in strawberry. |
| Key words: strawberry FaSKP1-1 cloning gene expression analysis |
