| 摘要: |
| 为探索SWEET基因家族在葡萄果实发育中的表达与功能，以本实验室完成的酿酒葡萄品种赤霞珠Ⅰ期和Ⅲ期果实的转录组数据为基础，筛选出在Ⅰ期和Ⅲ期表达量存在显著差异的9个SWEET基因家族成员，利用生物信息学工具，对这9个基因的基因结构、蛋白的基本理化性质、二级结构、亚细胞定位、保守基序和序列同源性等进行预测分析，并利用实时荧光定量PCR（qPCR）技术对分析结果进行验证。基因组定位结果发现这9个SWEET基因分布在7条染色体上，蛋白序列可分成4个亚族。不同成员间氨基酸数目、氨基酸序列间的疏水性存在一定的差异；二级结构预测结果显示，这9个SWEET基因的氨基酸序列以α-螺旋和无规则卷曲为主要组成部分；基因结构分析表明，除VvSWEET4含有4个内含子，其余8个均含有5个内含子；对9个SWEET基因家族成员蛋白的亚细胞定位预测分析表明：它们大多定位在质膜、叶绿体类囊体膜和液泡膜上；QPCR结果表明，在Ⅰ期和Ⅲ期差异表达的9个SWEET基因家族成员中，5个基因上调表达，4个基因下调表达，与转录组分析结果一致。 |
| 关键词: 葡萄 SWEET基因家族 生物信息学分析 QPCR |
| DOI：10.11841/j.issn.1007-4333.2016.11.04 |
| 投稿时间：2015-12-05 |
| 基金项目:国家自然科学基金项目（31171939） |
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| Bioinformatics analysis of differentially expressed SWEET genes between grape berry development stage Ⅰ and Ⅲ |
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WANG Zi-ran1, KUANG Liu-qing1, CHEN Shang-wu2, MA Hui-qin1
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| (1.College of Horticulture, China Agricultural University, Beijing 100193, China;2.College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China) |
| Abstract: |
| To explore SWEET gene family expression and function in grape berry,we 9 SWEETs,which were differentially expressed between grape berry development stage Ⅰ and Ⅲ by RNA-Seq data analyzes of Vitis vinifera L.cv.Cabernet Sauvignon were selected.By using bioinformatics approaches,their gene structure,protein physicochemical properties,secondary structures,subcellular localization,conserved motifs as well as sequence homology predicted and analyzed and real-time fluorescence quantitative PCR was used to validate some genes.Genetic mapping for SWEET genome localization found that they was distributed on seven chromosomes,9 VvSWEETs sequences was divided into four sub-families.Differences existed in the amino acid number and hydrophobic character among the 9 VvSWEETs.Secondary structure prediction revealed that the major composition of the amino acid sequences of the 9 VvSWEETs were alpha helix and random coil.Structural analysis of exons and introns showed that only VvSWEET4 has four introns,others have five introns.Protein subcellular localization analysis indicated that 9 VvSWEETs were localized in plasma membrane,chloroplast thylakoid membrane and the vacuole membrane.QPCR results indicated that the 9 SWEETs with differentially expression between grape berry development stage I and III,5 of which were up-regulated,and 4 down-regulated,which were consisting with transcriptome sequencing analysis. |
| Key words: grape berry SWEET gene family bioinformatics analysis QPCR |
