| 摘要: |
| 为研究禾谷镰刀菌侵染后玉米幼根内转录组的变化情况，利用RNAseq对禾谷镰刀菌接种后6和18 h的感病玉米自交系Y331分别进行转录组测序，分析接种后玉米幼根内差异表达的基因。结果表明：接种禾谷镰刀菌后，玉米幼根内共有5 153个基因差异表达；在接种后上调表达的聚类中，多种抗病过程相关的基因显著富集，包括苯丙烷类次生代谢物质合成过程的基因以及植物激素水杨酸（Salicylic acid，SA）、茉莉酸（Jasmonic acid，JA）和乙烯（Ethylene，ET）合成、响应及信号介导途径的基因；在下调表达的聚类中，植物生长发育、基础物质和能量代谢相关过程的基因显著富集。进一步分析发现：ET相关过程的基因在聚类1和聚类6中显著富集，均在接种后6 h前上调表达；苯丙烷类次生代谢物合成途径相关基因和镰刀烯醇毒素（Deoxynivalenol，DON）解毒相关基因在接种后上调表达。研究表明，SA、JA/ET、苯丙烷类次生代谢物和DON解毒基因在玉米和禾谷镰刀菌互作中发挥重要作用。 |
| 关键词: 玉米 转录组 禾谷镰刀菌 抗性 苯丙烷合成 激素 |
| DOI：10.11841/j.issn.1007-4333.2016.11.01 |
| 投稿时间：2016-02-17 |
| 基金项目:农业部948项目（2011-G15） |
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| Transcriptome analysis of maize root after inoculation of Fusarium graminearum |
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LIU Yong-jie, MA Chuan-yu, YE Jian-rong
|
| (College of Agronomy/National Maize Improvement Center of China, China Agricultural University, Beijing 100193, China) |
| Abstract: |
| Gibberella stalk rot,caused by Fusarium graminearum,is one of the most devastating soil-borne diseases in maize.A susceptible maize inbred line Y331 was inoculated with F.graminearum and sampled at 6 and 18 hours post inoculation for RNAseq.A total of 5 153 genes were differentially expressed after F.graminearum inoculation.Many defense related genes were significantly enriched in the upregulated clusters,while genes involved in growth and development,carbohydrate and ATP metabolism were enriched in the downregulated clusters.All these results indicate a cost-effective energy use of maize after pathogen inoculation.Genes involved in ET related processes were enriched in Cluster1 and 6,both of which were upregulated before 6 hours post inoculation.Biological processes like JA or SA biosynthesis process,response to JA or SA,JA or SA medicated signaling pathway were enriched in the upregulated clusters.In the upregulated clusters,genes involved in phenylpropanoid biosynthetic process were also enriched;genes involved in flavonoid and lignin biosynthesis and genes involved in DON detoxification were upregulated after F.graminearum inoculation.All these results indicate the important roles of ET/JA,SA and secondary metabolites in the interactions of maize and F.graminearum. |
| Key words: maize transcriptome Fusarium graminearum resistance phenylpropanoid biosynthetic process hormones |
