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| 基于表型性状和SSR标记的山西省青狗尾草资源遗传多样性分析 |
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张耀元1,2,3, 任彦鑫1, 禾璐1, 张雁明1, 张彬1, 王兴春4,3, 李红英4,2, 韩渊怀1,4,2,3
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| (1.山西农业大学 农学院, 山西 太谷 030801;2.农业部黄土高原作物基因资源与种质创制重点实验室, 太原 030031;3.杂粮种质资源发掘与遗传改良山西省重点实验室, 山西 太谷 030801;4.山西农业大学 农业生物工程研究所, 山西 太谷 030801) |
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| 摘要: |
| 利用表型性状和SSR标记对山西省41份青狗尾草资源进行遗传多样性分析。在表型水平，通过分析每份资源的株型、穗型、叶型等14个表型性状指标的测定结果，发现山西青狗尾草资源具有较为丰富的表型多样性，其中叶片绿色的深浅即叶绿素含量遗传多样性指数最高，为2.76，其余性状遗传多样性指数也大多保持在2.0左右；大多数青狗尾草的穗型为纺锤形，籽粒颜色和形状分别是青色和纺锤形。通过表型聚类可将山西青狗尾草资源划分为3个类群：第1类主要表现为株高、穗数等整体指标居中；第2类主要表现为植株小，穗数少，单株干重低等；第3类主要表现为植株高大，穗数多，穗重等。聚类分析发现表型多样性与山西特定生态地理区划有一定相关性。在分子水平，利用20对SSR引物共检测到102个等位变异，平均每个位点5个，基因多样性与PIC的平均值分别为0.66和0.61。虽然聚类分析、群体结构分析和主成分分析均将41份青狗尾草资源分为了3类，但3种方法对狗尾草资源的划分结果并不完全一致，且划分出的3个类群与山西的生态地理区划也不完全一致，表明不同表型和遗传背景的青狗尾草资源在山西混合分布，区域之间没有明显界限。 |
| 关键词: 青狗尾草 表型性状 SSR标记 遗传多样性 群体结构 |
| DOI：10.11841/j.issn.1007-4333.2016.10.02 |
| 投稿时间：2015-12-29 |
| 基金项目:国家自然科学基金项目（31371693，31471556，31471502）；教育部高等学校博士学科点专项科研基金项目（20131403110001）；山西农业大学科技创新基金项目（20142-07） |
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| Genetic diversity analysis of green foxtail germplasm resources in Shanxi Province based on phenotypes and SSR markers |
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ZHANG Yao-yuan1,2,3, REN Yan-xin1, HE Lu1, ZHANG Yan-ming1, ZHANG Bin1, WANG Xing-chun4,3, LI Hong-ying4,2, HAN Yuan-huai1,4,2,3
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| (1.College of Agriculture, Shanxi Agricultural University, Taigu 030801, China;2.Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau, Ministry of Agriculture, Taiyuan 030031, China;3.Shanxi Key Laboratory of Resources and Genetic Improvement of Minor Crops, Taigu 030801, China;4.Institute of Agricultural Bioengineering, Shanxi Agricultural University, Taigu 030801, China) |
| Abstract: |
| The genetic diversity of 41 green foxtail resources in Shanxi Province was investigated by phenotypes and SSR markers.At the phenotype level,14 character indicators such as the types of plant structure,shapes of panicle and leaf were determined.The results showed that the phenotypic diversity of Shanxi green foxtail resources were relatively abundant.The leaf color represented by chlorophyll content had the highest genetic diversity index (2.76),and the genetic diversity index for the rest of the characteristics were about 2.0.Most of the green foxtail had spindle panicle,with grains being cyan in color and spindle-shaped.These resources were divided into three groups by phenotypic clustering,and each group had its own characteristics:The first group showed average phenotypes among all the accessions including traits of plant height,panicle number,and etc;the second group mainly contained those of smaller plant height,fewer panicles and lower dry weight;the third group consisted mainly of those with bigger plant height,more panicles,lower panicle weight and etc.It was discovered that phenotypic diversity had some correlation with specific eco-geographical regions in Shanxi.At the molecular level,twenty pairs of SSR primers were selected to analyze the green foxtail resources and a total of 102 alleles were detected,with an average of 5 alleles per locus.The average value of gene diversity and polymorphism information content was 0.66 and 0.61,respectively.Although these resources were all divided into three categories by three different methods including clustering analysis,population structure analysis and principal component analysis,the classification of these resources were not completely consistent.Moreover,these three clusters were also inconsistent with the distribution of eco-geographical regions in Shanxi,indicating a partially mixed distribution of green foxtail resources in Shanxi and unclear boundary among regions. |
| Key words: Setaria viridis phenotypic traits SSR markers genetic diversity population structure |
