| 摘要: |
| 以优良植物生防菌解淀粉芽孢杆菌L-S60为研究对象，通过单因素试验研究酸碱、盐浓度、温度对该菌株生长的影响；检测解淀粉芽孢杆菌L-S60的产酶和抑菌特性；并研究优化解淀粉芽孢杆菌L-S60的固态发酵工艺。结果表明：解淀粉芽孢杆菌L-S60具有较宽的酸碱耐受性，盐的质量分数>10%时菌落数会显著下降，80℃以下菌落数无明显变化。解淀粉芽孢杆菌L-S60能产生蛋白酶、纤维素酶和淀粉酶，且对多种病原真菌有抑制作用。L-S60的最佳固体发酵条件为：温度35℃、接种量30%、料水质量比1：0.45、装瓶量40%和发酵时间42 h；最佳固体培养基组成为，m（花生饼粉）：m（麦麸）：m（棉粕）=53：35：12，外源物质蛋白胨、葡萄糖和MgSO4·7H2O添加量分别为3.00、2.00和0.20 g/kg，此时，解淀粉芽孢杆菌L-S60菌落数达到2.42×1010CFU/g。解淀粉芽孢杆菌L-S60具有很好的开发利用价值。 |
| 关键词: 解淀粉芽孢杆菌 生物学特性 固态发酵 菌落数 |
| DOI：10.11841/j.issn.1007-4333.2016.09.15 |
| 投稿时间：2015-08-13 |
| 基金项目:公益性行业（农业）科研专项经费项目 |
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| The biological characteristics and solid-state fermentation of Bacillus amyloliquefaciens L-S60 |
|
WANG Hui, YOU Cheng-zhen, QIN Yu-xuan, LI Ping-lan
|
| (College of Food Science and Nutrition Engineering, China Agricultural University, Beijing 100083, China) |
| Abstract: |
| The impacts of concentration of acid,alkali,salt and the temperature on the growth of Bacillus amyloliquefaciens L-S60 were investigated through single factor experiments.The biological features of L-S60 were studied and the solid-state fermentation condition of this strain was optimized in this study.The results showed that:The resistance of B.amyloliquefaciens L-S60 to acid and alkali is robust;the colony number of L-S60 dropped significantly when the salt concentration exceeded 10%;there was no significant difference of the colony number of L-S60 when the temperature is under 80℃.Strain L-S60 could produce amylase,protease and cellulose.It also showed strong inhibitory effects on a variety of pathogenic fungi.In order to make B.amyloliquefaciens L-S60 a good bio-fertilizer,the solid fermentation conditions and medium composition were also explored.The optimum temperature was 35℃,the inoculum concentration was 30% and the ratio of material and water was 1:0.45.We chose 40% as the amount of bottling and 42 h as the fermentation time.The best solid fermentation medium contained 53% of peanut shell powder,35% of wheat bran,and 12% of cottonseed meal.Finally,extra nitrogen source (3.00 g/kg),carbon source (2.00 g/kg),and inorganic salt (MgSO4.7H2O 0.20 g/kg) were added in the solid state fermentation medium.Under this condition,the number of B.amyloliquefaciens colonies L-S60 reached 2.42×1010 CFU/g. |
| Key words: Bacillus amyloliquefaciens biological characteristics solid-state fermentation the number of colonies |
