| 摘要: |
| 为探讨畜禽源大肠杆菌临床菌株产β-内酰胺酶及其基因特性,采用常规检测法检测菌株产β-内酰胺酶情况,PCR法检测ESBLs、AmpC基因型,质粒结合转移试验研究耐药基因分子传播机制,并使用微量肉汤稀释法检测供体菌及接合子的药物敏感性。结果显示:467株食品动物源大肠杆菌未检出产碳青霉烯酶和金属β-内酰胺酶,产ESBLs和AmpC分别达36.83%和13.70%;176株产β-内酰胺酶菌株检出blaTEM、blaCTX-M、blaOXA、blaCIT和blaDHA分别为84.09%、60.80%、14.77%、35.80%和1.70%;本次质粒转移率为50%,多数转化子获得了供体菌的耐药性及耐药基因。以上结果说明,产ESBLs、AmpC菌株广泛存在于本次检测菌株中,blaTEM、blaCTX-M和blaCIT是β-内酰胺酶基因主要流行基因型,质粒在β-内酰胺酶基因的水平传播起主要作用。 |
| 关键词: 畜禽 大肠杆菌 ESBLs AmpC 基因型 |
| DOI：10.11841/j.issn.1007-4333.2016.08.12 |
| 投稿时间：2015-10-15 |
| 基金项目:国家科技部支撑计划项目(2012BAC01B06);四川省科技厅项目-苗子工程(035Z1143) |
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| Detection of Escherichia coli plasmid-mediated β-lactamase from livestock and poultry and characterization of antimicrobial resistance |
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SHU Gang1, HOU Rong2, LIN Ju-chun1, ZHONG Qin-qing1, LIU Song-rui2, DENG Xiang-dong1
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| (1.College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China;2.Chengdu Research Base of Giant Panda Breeding, Chengdu 610081, China) |
| Abstract: |
| To detect and characterize plasmid-mediated β-lactamase in E.coli isolates derived from livestock and poultry, the prevalence of β-lactamase in E.coli isolates was investigated by conventional test.Genotypes of extended spectrum β-lactamases (ESBLs) and AmpC were detected by PCR;Genetic transmission of β-lactamase genes was carried by plasmid conjugation experiment;Antimicrobial susceptibility of donors and transconjugants was tested by using broth micro dilution method.The results showed that the detection rates of carbapenemase, metallo-β-lactamase, ESBLs and AmpC in 467 E.coli isolates were 0.00%, 0.00%, 36.83% and 13.70%, respectively;The prevalence rates of blaTEM, blaCTX-M, blaOXA, blaCIT and blaDHA in 176 β-lactamase positive strains were 84.09%, 60.80%, 14.77%, 35.80% and 1.70%, respectively;The positive rate of plasmid conjugation was 50.00% in the study, and the majority of transconjugants had acquired antimicrobial resistance phenotypes and genotypes originated from parental isolates.In conclusion, ESBLs or/and AmpC producing strains exist widely among the isolates tested, blaTEM, blaCTX-M and blaCIT are dominant genetypes, and horizontal plasmid transfer plays a major role in the spread of β-lactamase genes in E.coli. |
| Key words: livestock and poultry Escherichia coli extended spectrum β-lactamase AmpC genotype |
