| 摘要: |
| 为研究促性腺激素释放激素(GnRH)主动免疫对公猪肝脏脂肪代谢关键基因转录水平和酶含量的影响,选用36头公猪随机分为对照组、免疫组和手术组 (n=12),测定血清睾酮、甘油三酯、血糖及肝脏组织中脂肪代谢关键酶含量,实时荧光定量PCR分析肝脏组织关键基因mRNA表达变化。结果显示:GnRH主动免疫后公猪血清甘油三酯含量呈上升趋势,血清睾酮含量显著下降(P<0.05)。与对照组公猪相比,免疫去势显著提高肝脏脂肪酸合成酶(FAS)及乙酰辅酶A羧化酶(ACC)含量(P<0.05),且显著提高肝脏FAS、ACC、微粒体甘油三脂转运蛋白(MTTP)基因mRNA表达水平(P<0.05),显著下调激素敏感酯酶(HSL)与过氧化物酶体增殖物激活型受体γ(PPAR γ)基因mRNA表达水平(P<0.05)。但免疫公猪血清甘油三酯含量及肝脏FAS、ACC、固醇调节元件结合蛋白-1c(SREBP-1c)mRNA表达仍显著低于手术去势公猪相应指标(P<0.05)。上述结果表明,GnRH主动免疫提高了公猪肝脏脂肪合能力,但其脂肪合成能力仍低于传统手术去势公猪。 |
| 关键词: 促性腺激素释放激素 主动免疫 肝脏 脂肪代谢 公猪 |
| DOI：10.11841/j.issn.1007-4333.2016.08.10 |
| 投稿时间：2015-09-21 |
| 基金项目:四川省教育厅重点项目(15ZA0005);四川农业大学双支计划(00770107) |
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| Effects of active immunization against GnRH onhepatic lipid metabolism in male pig |
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WANG Zuo-wei, HAN Xing-fa, DU Xiao-gang, ZENG Xian-yin, CAO Xiao-han
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| (College of Life Science, Sichuan Agricultural Unicersity, Ya'an 625014, China) |
| Abstract: |
| Thirty-six male pigs were randomly allocated to three experimental groups (n=12):immunocastrates, surgical castrates and intact controls.Serum concentrations of testosterone, triglyceride and glucose and lipid metabolism enzyme in the liver were determined.The mRNA expressions of metabolism-related genes in the liver were quantified by real-time fluorescence quantitative PCR technique.The results showed that:Active immunization against GnRH increased serum triglyceride, significantly reduced serum testosterone (P<0.05);Compared with intact controls, immunocastration of male pig significantly increased the capacity of fatty acid syntheses (FAS) and acetyl CoA-carboxylase (ACC) (P<0.05), and mRNA expression levels of FAS, ACC and microsomal triglyceride transfer protein (MTTP) (P<0.05); However, significantly reduced the levels of hormone sensitive lipase (HSL) and peroxisome proliferator-activated receptor γ (PPAR γ) in the liver (P<0.05) while the serum triglyceride concentrations and hepatic FAS, ACC and sterol regulatory element binding proteins-1c (SREBP-1c) mRNA levels in immunocastrates were still significantly lower than in surgical castrates.Taken together, active immunization against GnRH improves the capacity of fat synthesis in the liver, but which is still remained at a lower level than that of surgical castrates. |
| Key words: GnRH active immunization liver lipid metabolism male pig |
