| 摘要: |
| 为研究水杨酸(SA)对‘赤霞珠’葡萄果皮中总类黄酮和白藜芦醇含量的影响。在花后10 d,使用25、50、100 mg/L的水杨酸(SA)对‘赤霞珠’葡萄果穗进行喷施处理,以喷施清水为对照,在果实发育过程中测定葡萄果皮白藜芦醇和总类黄酮的含量,分析不同浓度的水杨酸(SA)处理对果皮中总类黄酮和白藜芦醇含量及相关酶活性的影响。在赤霞珠葡萄发育过程中,果皮中总类黄酮的含量在花后35 d最低,之后开始上升,直至成熟。白藜芦醇含量呈双峰型变化趋势,峰值分别出现在花后35和80 d。且经不同浓度水杨酸处理后,总类黄酮和白藜芦醇含量均得到提高,其中分别以25和50 mg/L水杨酸处理效果最好。与总类黄酮和白藜芦醇合成密切相关的苯丙氨酸解氨酶(PAL)、肉桂酸4-羟基化酶(C4H)、4-香豆酸辅酶A连接酶(4CL)活性随果实发育呈双峰型变化。相关性分析表明,白藜芦醇合成积累的关键酶是C4H、4CL。由此推断,外源水杨酸(SA)参与并调节了‘赤霞珠’葡萄果皮总类黄酮和白藜芦醇含量的积累及相关酶活性的变化。 |
| 关键词: 葡萄 水杨酸 总类黄酮 白藜芦醇 酶 |
| DOI：10.11841/j.issn.1007-4333.2016.07.05 |
| 投稿时间：2015-09-22 |
| 基金项目:国家自然科学基金资助项目(31260456) |
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| Effects of salicylic acid on the contents of total flavonoids and resveratrol and related enzyme activities in ‘Cabernet Sauvignon’ |
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DAI Hong-jun, QIN Chen-liang, DING Ling
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| (College of Agriculture/Grape and Wine Engineering Research Center for Education Ministry, Ninxia University, Yinchuan 750021, China) |
| Abstract: |
| To investigate the influences of exogenous salicylic acid (SA) treatment on the contents of total flavonoids and resveratrol in Vitis vinifera cv.Cabernet Sauvignon grape berry, the grape clusters of Cabernet Sauvignon were sprayed with 0 (control), 25, 50, or 100 mg/L SA at 10 days after anthesis, respectively.The contents of total flavonoids and resveratrol in grape skins at different periods of fruit ripening were determined to analyze the effects of SA on total flavonoids, resveratrol accumulation and related enzyme activies.The total flavonoids content were minimum after flowering 35 days in grape skins, then increased dramatically to full maturity during fruit development.Resveratrol presented double peak during grape ripening, one at 35 days, another at 80 days after anthesis. SA treatment increased the total flavonoids and resveratrol in grape of Cabernet Sauvignon, 25 and 50 mg/LSA treatment was significantly different from the control.The activity of phenylalanine ammonia lyase (PAL), cinnamic acid 4-hydroxylase (C4H), 4-coumaric acid coenzyme A ligase (4CL) were closely related to synthesis of total flavonoids and resveratrol presented bimodal changes with the development of the fruit.The result showed that exogenous SA participated in regulating the accumulated content of total flavonoids, resveratrol and the activity of related enzymes of phenylpropanoid metabolis pathway. |
| Key words: grape SA total flavonoids resveratrol enzyme |
