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| 授高油玉米花粉对普通玉米籽粒蛋白质积累及氮代谢相关酶活性的影响 |
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夏光利1, 董浩1,2, 宋绪鹏3, 毕军1, 朱国梁1, 陈靖3
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| (1.泰安市农业科学研究院, 山东 泰安 271000;2.山东农业大学 作物生物学国家重点实验室, 山东 泰安 271000;3.泰安市岱岳区农业局, 山东 泰安 271000) |
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| 摘要: |
| 将高油玉米花粉给普通玉米授粉,研究杂交组合籽粒中蛋白质含量和氮代谢相关酶活性的变化情况。试验结果表明,与普通玉米自交相比,授高油玉米花粉的普通玉米籽粒中的蛋白质含量增加。高油玉米花粉提高了普通玉米叶片中硝酸还原酶和籽粒中谷氨酸脱氢酶的活性,籽粒中谷胺酰胺合成酶和谷氨酸合成酶的活性也有不同程度地提高。这种影响导致了杂交植株与普通玉米自交植株之间蛋白质合成能力的不同。 |
| 关键词: 高油玉米 花粉 蛋白质含量 氮代谢相关酶 |
| DOI：10.11841/j.issn.1007-4333.2016.07.02 |
| 投稿时间：2015-12-15 |
| 基金项目:山东省科技发展计划项目(2014GNC113001);作物生物学国家重点实验室开放课题(2014KF11) |
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| Influence of high-oil maize pollen to normal maize kernel protein accumulation and nitrogen metabolism related enzymes activities |
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XIA Guang-li1, DONG Hao1,2, SONG Xu-peng3, BI Jun1, ZHU Guo-liang1, CHEN Jing3
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| (1.Tai'an Academy of Agricultural Sciences, Tai'an 271000, China;2.State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an 271000, China;3.Daiyue Agricultural Bureau in Tai'an, Tai'an 271000, China) |
| Abstract: |
| Changes of protein accumulation and nitrogen metabolism related enzymes activities in the process of grain formation were studied by pollinating normal maize with high-oil corn's pollen.The results showed that the content of protein was higher in normal corn pollinated with high-oil corn's pollen than that of self-fertilized kernel;The xenia effect of high-oil corn did improve the nitrate reductase activity in the ear leaves and the glutamate dehydrogenase activity in normal corn kernel.There were different degrees of impact on the activities of glutamine synthase and glutamate synthase between the hybridization kernel and the self-fertilized kernel resulting in the difference of the competence of protein synthesizing between normal corn pollinated with high-oil corn's pollen and the corresponding self-fertilized normal corn. |
| Key words: high-oil maize pollen protein content nitrogen metabolism related enzymes |
