| 摘要: |
| 为了解固氮类芽孢杆菌对小麦的增产效果,对实验室分离保存的4株固氮类芽孢杆菌菌株1-18、1-33、1-43 及1-49,在固氮酶活性、吲哚乙酸(IAA)分泌量及接种小麦后的增产效果进行了比较。结果表明,这4株固氮类芽孢杆菌接种小麦后,比不接种固氮菌剂的对照增产5.1%~26.9%,其中菌株1-18的增产效率最高(增产26.9%),其次是菌株1-49(增产18.7%)。相应地,这2株菌株的固氮酶活性也比其他2株菌株高,菌株1-18固氮酶活性为1 043.6 nmol/(mg·h),菌株1-49为969.5 nmol/(mg·h),但分泌的IAA量低于其他2个菌株,菌株1-18 分泌IAA量为24.95 μg/mL,菌株1-49分泌IAA量为26.34 μg/mL。结果说明用于本研究的固氮芽孢杆菌在固氮酶活性、IAA分泌量及对小麦的增产效果方面存在差异。 |
| 关键词: 固氮类芽孢杆菌 小麦产量 固氮酶活性 吲哚乙酸 |
| DOI：10.11841/j.issn.1007-4333.2016.06.006 |
| 投稿时间：2015-08-31 |
| 基金项目:国家"863"项目资助(2013AA102802-04) |
|
| Effect of nitrogen-fixing Paenibacillus spp.on wheat yield |
|
SHI Hao-wen1, LI Yong-bin1, LI Peng-fei1, WANG Zhi-min2, CHEN San-feng1
|
| (1.College of Biological Sciences, China Agricultural University, Beijing 100193, China;2.College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China) |
| Abstract: |
| Four nitrogen-fixing Paenibacillus strains 1-18, 1-33, 1-43 and 1-49 are used to inoculate wheat, and the wheat yields are analyzed to determine the effect of these strains on wheat yields.The results showed that wheat yields were increased by 26.9%, 9.7%, 5.1% and 18.7%, respectively.Furthermore, the nitrogenase activities and IAA amounts produced by these 4 strains were analyzed.Of these 4 strains, strain 1-18 exhibited the highest nitrogenase activity (1 043.6 nmol/(mg·h)) and the activity (969.5 nmol/(mg·h)) of strain 1-49 is higher than the rest 2 strains 1-33 ((565.9±3.0) nmol/(mg·h)) and 1-43 ((904.5±4.3) nmol/(mg·h)).However, the IAA amounts produced by strains 1-18 ((24.95±1.2) μg/mL) and 1-49 ((26.34±4.2) μg/mL) are lower than those produced by strains 1-33 ((27.73±1.8) μg/mL) and 1-43 ((49.91±3.6) μg/mL).Our study provided fundamental information for strain improvement of nitrogen-fixing strain for microbial fertilizer. |
| Key words: nitrogen-fixing Paenibacillus wheat yield nitrogenase activity IAA |
