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| 双价抗寒基因CBF3/COR15A转化大白菜的初步研究 |
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孟平红1, 李桂莲1, 李苍山2, 蔡霞1, 郭惊涛1, 邓英1, 张兴国3
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| (1.贵州省园艺研究所, 贵阳 550006;2.四川省巴中市第二中学, 四川 巴中 636600;3.西南大学 园艺园林学院, 重庆 400715) |
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| 摘要: |
| 为探索拟南芥的冷诱导转录因子 CBF3 和抗寒基因 COR15A 在"黔白"系列大白菜的转化体系,以"黔白"系列大白菜3个优良自交系作为研究对象,分别用大白菜4 d苗龄的带柄子叶、半子叶、下胚轴作为外植体,利用农杆菌介导的方法将同时含有 CBF3 和 COR15A 双价抗寒基因的表达载体转入大白菜,就影响大白菜再生和遗传转化的因素进行研究。通过抗生素筛选得到大白菜T0代转基因抗性植株36株,经PCR检测后获得转基因阳性植株6株。经抗寒性初步鉴定,转基因大白菜植株抗寒性超过对照。 |
| 关键词: 大白菜 遗传转化 抗寒基因 CBF3 抗寒基因 COR15A |
| DOI：10.11841/j.issn.1007-4333.2016.06.005 |
| 投稿时间：2015-07-18 |
| 基金项目:贵州省优秀科技教育人才省长专项资金项目(黔省专合字[2009]61号);贵州省农科院人才启动项目(黔农科院[2009]002号);国家自然科学基金资助项目(31160238);国家星火计划重大项目(2012GA820001);贵州省科技重大专项(黔科合重大专项字[2011]6001及重大专项字[2013]6061-1)、贵州省重点攻关(黔科合成转字[2015]5022A号) |
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| Preliminary research of the transformation of bivalent cold-resistance genes CBF3/COR15A into Chinese cabbage |
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MENG Ping-hong1, LI Gui-lian1, LI Cang-shan2, CAI Xia1, GUO Jing-tao1, DENG Ying1, ZHANG Xing-guo3
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| (1.Guizhou Horticulture Institute, Guiyang 550006, China;2.Bazhong Second School of Sichuan, Bazhong 636600, China;3.College of Horticulture and Landscape Achitecture, Southwest University, Chongqing 400715, China) |
| Abstract: |
| To establish "Qianbai" series cabbage regeneration-transforming technology system of cold resistance gene CBF3 and COR15A, "Qianbai" series 3 inbred lines of Chinese cabbage were used as the research object.Using the cotyledons of petiole, half leaf, hypocotyl of 4 d age seedlings of Chinese cabbage as explants, and the cotyledon petiole of Zui4 as the receptor of genetic transformation, we transferred the expression vector with bivalent cold resistance gene CBF3 and COR15A into Chinese cabbage by agrobacterium-mediated method.Factors affecting explants regeneration and genetic transformation of Chinese cabbage were also studied.By antibiotic selection, we obtained 6 transgenic plants by PCR from 36 T0 transgenic plants.Preliminary identification of cold resistance showed that the cold resistance of transgenic cabbage plants is higher than that of control plants. |
| Key words: Chinese cabbage transformation cold-resistance genes CBF3 COR15A |
