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| 基于表型和SRAP标记的唐菖蒲品种遗传多样性分析 |
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刘晨1, 高明伟2, 刘超1, 何俊娜1, 吴健1, 僧珊珊1, 钟雄辉1, 吴晨雨1, 张凤勤1, 义鸣放1
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| (1.中国农业大学 农学与生物技术学院/花卉发育与品质调控北京市重点实验室, 北京 100193;2.北京市花木有限公司, 北京 100093) |
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| 摘要: |
| 为进一步研究唐菖蒲杂交育种亲本选配和杂种的早期鉴定,以21个唐菖蒲品种为试材,对这些品种的表型性状特征进行聚类分析,并利用SRAP技术对其构建指纹图谱。结果表明:21个品种的表型性状表现出一定的遗传多样性,变异6.78%~147.29%。经过对SRAP-PCR反应条件的优化,从100对引物中筛选出16对引物适用于唐菖蒲PCR反应;通过对21个品种SRAP标记的试验与分析,共在468个位点上扩增出条带,多态性位点411个,平均每个引物组合扩增多态性条带25.7个;基于SRAP标记的UPGMA 聚类分析显示,21个品种间Jaccardp's 相似系数0.46~0.75。将2种聚类结果进行比较发现,唐菖蒲品种的分类与表型性状无显著相关性,说明其品种具有复杂的遗传背景。 |
| 关键词: 唐菖蒲 表型性状 SRAP标记 |
| DOI：10.11841/j.issn.1007-4333.2016.05.008 |
| 投稿时间：2015-04-03 |
| 基金项目:国家自然科学基金资助项目(31171991);国家教委博士点专项基金(20120008110006) |
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| Genetic diversity analysis of Gladiolus cultivars based on morphology and SRAP markers |
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LIU Chen1, GAO Ming-wei2, LIU Chao1, HE Jun-na1, WU Jian1, SENG Shan-shan1, ZHONG Xiong-hui1, WU Chen-yu1, ZHANG Feng-qin1, YI Ming-fang1
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| (1.Collage of Agronomy and Biotechnology/Beijing Key Laboratory of Development and Quality Control of Ornamental Crops, Department of Ornamental Horticulture and Landscape Architecture, China Agricultural University, Beijing 100193, China;2.Beijing Flowers and Trees Co., LTD, Beijing 100093, China) |
| Abstract: |
| For parent selection and early identification of hybrids in cross breeding of Gladiolus hybridus, classification and genetic relationship of 21 G.hybridus cultivars were studied by phenotypic characters.SRAP fingerprints of these cultivars were also established.The results showed that:These cultivars displayed a certain degree of genetic variation, ranged between 6.78% and 147.29%, in morphology.Sixteen primer pairs were selected from 100 primers pairs after SRAP-PCR reaction optimization.Twenty-one cultivars were assayed by SRAP method, and a total of 411 polymorphic DNA fragments were detected among 468 amplified fragments, with an average of 25.7 fragments for each primer pairs.Cluster analysis based on SRAP markers showed that the Jaccardp's similarity coefficients of these 21 G.hybridus cultivars were ranged from 0.46 to 0.75.There was no significant correlation between the classification and phenotypic characters suggesting that cultivars of G.hybridus have complex genetic basis. |
| Key words: Gladiolus hybridus phenotypic characters SRAP marker |
