| 摘要: |
| 为研究生长素结合蛋白(Auxin binding protein 1 ABP1)与不同苹果砧木缺铁胁迫的关系,通过对铁高效/铁低效苹果基因型苹果砧木在不同铁素浓度下 abp1 的转录和翻译水平进行检测,结果表明:铁高效苹果基因型的 abp1 的转录和翻译水平明显高于铁低效苹果基因型;进而依托拟南芥的不同类型(生态型、突变体型和突变体恢复型)植株,通过检测它们的缺铁应答相关生理指标和铁吸收转运相关基因表达的变化差异,发现拟南芥 abp1 的突变引发了根三价铁还原酶活性明显升高,根毛数量明显增加,且 IRT1、FRO2 和FIT基因明显上调表达。综上结果表明ABP1通过影响生长素运输进而影响了植物缺铁胁迫应答反应。 |
| 关键词: 苹果 砧木 拟南芥 缺铁胁迫 ABP1 |
| DOI：10.11841/j.issn.1007-4333.2016.05.005 |
| 投稿时间：2015-03-31 |
| 基金项目:国家自然基金资助项目(31272139);农业部园艺作物营养与生理重点实验室;北京市果树逆境生理与分子生物学重点实验室资助 |
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| Research of ABP1 participating in iron-deficiency stress response in apple stocks |
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ZHANG Meng-meng, XU Xue-feng, WU Ting, ZHANG Xin-zhong, HAN Zhen-hai, WANG Yi
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| (College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China) |
| Abstract: |
| Our research aims to explore the relationship between auxin binding protein 1(ABP1) and iron-deficiency stress response.Firstly, we tested the transcriptional and translocational of abp1 of the apple stocks (iron-efficient/iron-sensitive apple stock) under the iron-deficiency and iron-sufficiency conditions.The results showed that the expression level of transcriptional and translocational abp1 in iron-efficient apple stock was higher than in the iron-sensitive one.We tested variations of the iron relative physiological indexes and expression of the iron absorption and transportation related genes (FIT, FRO2 and IRT1) in Columbia, abp1 mutant and mutant recovery Arabidopsis plants.abp1 mutant (abp1-5) displayed higher Fe(III) reductase activity, increased root hair numbers, and obviously higher gene expression level of FIT, FRO2 and IRT1.In conclusion, ABP1 responds to the iron-deficiency stress by affecting the transportation of auxin. |
| Key words: apple stock Arabidopsis iron-deficiency ABP1 |
