| 摘要: |
| 以携带草莓斑驳病毒(Strawberry mottle virus)的'红颜'草莓为材料,通过对玻璃化超低温脱毒技术中各程序的优化,建立适合'红颜'草莓的茎尖玻璃化超低温脱毒体系。结果表明:1~2 mm的茎尖在0.5 mol/L蔗糖的预培养基预培养3~7 d,室温装载处理60 min,0 ℃玻璃化处理180 min,液氮冷冻60 min,40 ℃解冻2 min,高糖溶液卸载20 min后接种到MS+2.0 mg/L 6-BA再生培养基上,成活率可达70%以上。再生培养60 d后,随机选取20株再生植株,利用RT-PCR技术检测草莓斑驳病毒的脱毒效果,脱毒率为100%。利用该体系可以克服传统茎尖脱毒受茎尖大小的限制(0.1~0.3 mm),并有效脱除草莓斑驳病毒。 |
| 关键词: 草莓 超低温 脱毒 草莓斑驳病毒 病毒检测 |
| DOI：10.11841/j.issn.1007-4333.2016.03.08 |
| 投稿时间：2015-04-22 |
| 基金项目:科技部科技支撑计划(213BAD201301); 国家"863"计划(2013AA103002) |
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| Preliminary study on elimination of Strawberry mottle virus (SMoV) from shoot tips by vitrification-cryopreservation treatment |
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SHENG Hong-ya, WAN Ji-hua, XU Chuan, WANG Hong-qing
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| (College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China) |
| Abstract: |
| Strawberry can be infected by virus easily, which lead to yield and quality losses.'Benihoppe' strawberry (Fragaria×ananassaDuch) infected with strawberry mottle virus was used as materials.By optimizing the parameters of vitrification-cryopreservation, appropriate cryopreserved detoxification of Benihoppe shoot tips was established.The results indicated:The shoot tips(1-2 mm) were pre-cultured on basic medium with 0.5 mol/L sucrosef for 3 to 7 days, then the pre-cultured shoot tips were loaded for 60 min at 25 ℃ and vitrified for 180 min at 0 ℃, immersed in liquid nitrogen for 1h, and rewarmed in water for 2 min at 40 ℃.After uninstalling in high sugar solution for 20 min, the shoot tips were inoculated into regeneration medium.In the end, the percentage of survived shoot tips was more than 70%.Twentyregeneration plants were chosen to test the effect of SMoV elimination.The result showed that elimination rate of SMoV reached 100%. |
| Key words: strawberry cryopreservation viruselimination Strawberry mottle virus virus detection |
