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| 红麻细胞质雄性不育系UG93A和保持系UG93B cox1的克隆和表达分析 |
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廖小芳1,2,3, 刁勇1,2, 邱爱华1,2, 赵艳红4, 周步进1,2, 陈鹏1,2, 周瑞阳1,2
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| (1.广西大学植物遗传育种重点实验室, 南宁530005;2.广西大学农学院, 南宁530005;3.广西大学生命科学与技术学院, 南宁530005;4.广西壮族自治区农业科学院经济作物研究所, 南宁530007) |
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| 摘要: |
| 利用hiTAIL-PCR技术扩增红麻 cox1 全长及侧翼序列,结果表明,在红麻不育系UG93A和保持系UG93B中分别获得长度为2 149和3 244 bp的序列,包含 cox1 基因CDS全长及其5'和3'部分侧翼序列,通过分析得出不育系和保持系中 cox1 的CDS序列长度为1 602 bp,编码533个氨基酸残基,分子量为58.31 ku。BLAST 序列比对发现,UG93A 和 UG93B cox1 的CDS序列虽然存在4个碱基差异,但推导的氨基酸序列一样,并且UG93A和UG93B cox1 两端侧翼序列结果一致。RNA Blot分析显示 cox1 基因在红麻UG93A、UG93B和F1(UG93A/992)的转录本大小基本相同,推测 cox1 基因不是导致红麻CMS(cytoplasmic male sterility)的直接因子。qRT-PCR结果表明,UG93A中 cox1 的表达量显著低于 UG93B和F1(UG93A/992),推测 cox1 在红麻花粉发育能量代谢过程中有着重要的作用。 |
| 关键词: 红麻 细胞质雄性不育系和保持系 cox1 表达分析 |
| DOI：10.11841/j.issn.1007-4333.2016.03.06 |
| 投稿时间：2015-04-22 |
| 基金项目:国家自然科学基金(31171600); 广西优秀博士学位论文培育项目(T3310098603) |
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| Cloning and expression analysis of cox1 in cytoplasmic male sterility line UG93A and its maintainer line UG93B of kenaf |
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LIAO Xiao-fang1,2,3, DIAO Yong1,2, QIU Ai-hua1,2, ZHAO Yan-hong4, ZHOU Bu-jin1,2, CHEN Peng1,2, ZHOU Rui-yang1,2
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| (1.The Key Laboratory of Plant Genetic Breeding, Guangxi University, Nanning 530005, China;2.College of Agriculture, Guangxi University, Nanning 530005, China;3.College of Life Science and Technology, Guangxi University, Nanning 530005, China;4.Cash Crops Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China) |
| Abstract: |
| Full-length CDS including its flanking sequence of cox1 cloned by hiTAIL-PCR.They were 2 149 and 3 244 bp in cytoplasmic male sterile line UG93A and its maintainer line UG93B, respectively, both contained an 1 602 bp opening reading frame, encoding 533 AA residue with 58.31 ku molecular weight.Four single base variations were found through BLAST analysis, but the deduced amino acid sequence were the same as well as their flanking sequences.RNA blotting results suggested that there was no size difference of mRNA among UG93A, UG93B and F1 (UG93A/992).qRT-PCR analysis revealed that the expression level of cox1 was reduced significantly in UG93A compared to UG93B and F1 (UG93A/992), indicating that cox1 might play an important role in energetic metabolism during anther development of kenaf. |
| Key words: kenaf cytoplasimic sterile line and its maintainer line cox1 expression analysis |
