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不同pH对蓝莓组培苗生长和元素吸收的影响
曹增强, 徐莹莹, 张宁, 贾文锁, 张潞生
0
(中国农业大学农学与生物技术学院, 北京 100193)
摘要:
为探究pH对蓝莓生长和元素吸收的影响,以非生根蓝莓组培苗为试验材料,通过设置不同pH梯度进行研究。结果表明:当pH小于或大于对照(pH 5.4)时,蓝莓组培苗的鲜重和干重都会相应地减小;pH过低(4.5)或过高(7.0)则会抑制蓝莓组培苗的分枝和株高,植株叶片变小,叶片颜色变化显著。适当提高pH水平(6.0和6.5)会促进蓝莓组培苗产生分枝,但叶片会变小、变黄或变淡紫。pH过高(6.0、6.5和7.0),抑制蓝莓组培苗对Cu、Zn、P、K和Mn元素的吸收,而当pH过低(4.5)时,则会抑制蓝莓组培苗对Cu、P、K、Mg和Mn元素的吸收。过低或过高的pH水平都会促进蓝莓植株组织和细胞对Na元素的吸收。培养基pH为7.0时,则会促进蓝莓组培苗对Fe、Ca和B的吸收。
关键词:  蓝莓  pH  组培苗  营养元素
DOI:10.11841/j.issn.1007-4333.2016.02.06
投稿时间:2015-03-20
基金项目:教育部博士点基金项目(20110008130003); 农业部公益性项目(201003064)
Effects of different pH levels on the growth and mineral absorption of blueberry tissue culture plantlets
CAO Zeng-qiang, XU Ying-ying, ZHANG Ning, JIA Wen-suo, ZHANG Lu-sheng
(College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China)
Abstract:
This study was undertaken to determine the effect of pH on the growth and mineral absorption of the non-rooting tissue culture plantlets of blueberry.The results showed that:The fresh and dry weight of blueberry tissue culture plantlets decreased with decreasing of pH levelwhen the pH was lower than the control treatment and decreasedwith increasing of pH level when the pH was higher than the control treatment;the number of branches and the height were significantly restricted,leaves became smaller and the color of leaves changed when the pH was 4.5 or 7.0;the number of branches was promoted but the leaves became smaller and the color became yellowish and lavender when the pH was 6.0 and 6.5;the absorption of Cu,Zn,P,K and Mn was restricted when the pH were 6.0,6.5 and 7.0;the absorption of Cu,P,K,Mg and Mn was also restricted when the pH was 4.5;the absorption of Na was promoted whether the pH was low(4.5 and 5.0)or high (6.0,6.5 and 7.0);and the absorption of Fe,Ca and B was promoted when the pH was 7.0.
Key words:  blueberry  pH  tissue culture plantlets  elements