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| 玉米单倍体自然二倍化研究 |
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吴鹏昊1,2, 任姣姣1, 田小龙1, 王乐乐1, 李国梁1, 刘文欣1, 陈绍江1
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| (1.中国农业大学农学与生物技术学院/国家玉米改良中心/北京作物遗传改良重点实验室, 北京 100193;2.新疆农业大学农学院, 乌鲁木齐 830052) |
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| 摘要: |
| 本研究以玉米杂交种郑单958诱导产生的玉米单倍体群体为材料,利用流式细胞仪对单倍体自然条件下的二倍化现象进行了观察。结果表明,在自然条件下,体细胞加倍与生殖细胞加倍并不显著相关,雌雄穗加倍存在差异,并且雌穗自然加倍率较高。雄穗可育单倍体植株的体细胞基本上处于单倍状态(1C),少数体细胞中存在单倍体与二倍体细胞的倍性嵌合现象,并检测到了1%~2%的非整倍体(1.5C)现象。体细胞二倍化有随雄穗育性水平升高而增加的趋势。 |
| 关键词: 流式细胞仪 玉米 单倍体 自然加倍 二倍化 |
| DOI：10.11841/j.issn.1007-4333.2016.01.01 |
| 投稿时间：2015-06-08 |
| 基金项目:现代农业产业技术体系(CARS-02-09);国家"863"计划项目(2011AA10A103) |
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| Studies on haploid spontaneous diploidization in maize |
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WU Peng-hao1,2, REN Jiao-jiao1, TIAN Xiao-long1, WANG Le-le1, Li Guo-liang1, LIU Wen-xin1, CHEN Shao-jiang1
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| (1.College of Agronomy and biotechnology/National Maize Improvement Center of China/Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, China;2.College of Agronomy, Xinjiang Agriculture University, Urumqi 830052, China) |
| Abstract: |
| Based on the haploid population from maize hybrid Zhengdan958,flow cytometer was used to observe the haploid spontaneous diploidization.The results showed that in the natural condition,there was no significant correlation between germ cell and somatic cell,and difference in diploidization between tassels and ears,which was found higher than tassels.Most of male fertile haploids were detected to stay in the state without diploidization(1C),only a few fertile haploids had been found to be the chimera,and there were about 1%-2% fertile haploids being detected to have aneuploidy(1.5C).There was a trend that somatic cell doubling increased with haploid fertility raised. |
| Key words: flow cytometer maize haploid spontaneous doubling diploidization |
