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| 番茄SlMYB12RNAi表达载体构建及功能的初步研究 |
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刘琳琳1, 田荣荣2, 郭萧1, 廖雄1, 王爽1, 姚丽萍1, 赵迪1, 王彦涛1, 李天红1
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| (1.中国农业大学 农学与生物技术学院, 北京 100193;2.北京城市学院 生物医药学部, 北京 100194) |
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| 摘要: |
| 以Micro-Tom番茄(Solanum lycopersicum L)为材料,构建 SlMYB12 RNAi载体,利用农杆菌介导法导入番茄;对转基因植株评价发现, SlMYB12 基因被干扰后植株出现了植株矮化、果实变小、种子数减少和转色期提前等变化;实时荧光定量PCR检测表明干扰植株果实中类黄酮路径相关基因 SlCHS、SlF3H、SlF3'H 表达量下降,SlCHI基因表达量上升。 |
| 关键词: 番茄 类黄酮 SlMYB12 RNAi 实时荧光定量PCR |
| DOI：10.11841/j.issn.1007-4333.2015.06.15 |
| 投稿时间：2015-02-05 |
| 基金项目:国家自然科学基金(31171938); 公益性行业(林业)科研专项经费(201404720) |
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| Construction of SlMYB12 RNAi expression vector and functional analysis in tomato |
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LIU Lin-lin1, TIAN Rong-rong2, GUO Xiao1, LIAO Xiong1, WANG Shuang1, YAO Li-ping1, ZHAO Di1, WANG Yan-tao1, LI Tian-hong1
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| (1.College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China;2.School of Biomedicine, Beijing City University, Beijing 100194, China) |
| Abstract: |
| Micro-Tom tomato (Solanum lycopersicum L.) was used as material in this study.The SlMYB12 RNAi expression vector was constructed,and introduced into tomato via Agrobacterium tumefaciens mediated method.PCR results showed that the gene was integrated in tomato.The transgenic lines exhibited dwarf phenotype,smaller fruits and advanced mature period.The results of qRT-PCR showed that the expression of flavonoid biosynthesis-related genes SlCHS、SlF3H、SlF3'H were decreased in transgenic lines,while the expression of SlCHI gene was increased.These results provided an important basis for further study of SlMYB family genes function. |
| Key words: tomato flavonoid SlMYB12 RNA interference qRT-PCR |
