| 摘要: |
| 为评价浓缩乳蛋白(Milk protein concentrate)溶液经动态高压微射流(Dynamic high-pressure microfluidization,DHPM)及DHPM结合加热(65℃ 30 min和95℃ 30 min)处理过程中α-乳白蛋白、β-乳球蛋白、α-酪蛋白和β-酪蛋白抗原性的变化,采用间接竞争ELISA法测定不同压力条件下MPC中各蛋白抗原性的变化。结果表明:1)4种蛋白抗原性对DHPM及DHPM结合加热的反应各不相同。2)DHPM+65℃/95℃处理后,蛋白质α-乳白蛋白、α-酪蛋白和β-酪蛋白抗原性均明显低于DHPM处理样和水化对照样。因此,DHPM及DHPM结合加热处理可降低MPC中酪蛋白的抗原性,增加α-乳白蛋白的抗原性,但对β-乳球蛋白效果不明显。 |
| 关键词: 动态高压微射流 加热 浓缩乳蛋白 抗原性 |
| DOI：10.11841/j.issn.1007-4333.2015.05.30 |
| 投稿时间：2014-12-15 |
| 基金项目:国家自然科学基金项目(31171715,30871817) |
|
| Effects of dynamic high-pressure microfluidization on antigenicity of milk protein concentrate |
|
XU Qian1,2, LI Da-peng1, HUANG He-ping1, SHI Jing1, LUO Yong-kang1
|
| (1.College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China;2.Production & Construction Group Key Laboratory of Special Agricultural Products Further Processing in Southern Xinjiang, Alar 843300, China) |
| Abstract: |
| Milk protein concentrates were treated by dynamic high-pressure microfluidization (DHPM) and DHPM combined with heat treatment (65℃,95℃ for 30 min).Indirect competitive ELISA method was used to determine the antigenicity of α-lactalbumin, β-lactoglobulin, α-casein and β-casein in MPC under different conditions.The results showed that the reactions of four antigenic proteins to DHPM and DHPM combined with heat treatment were not identical.The antigenicity of α-lactalbumin、 α-casein and β-casein were significantly lower than DHPM treatment samples and hydrating sample. |
| Key words: dynamic high-pressure microfluidization heat treatment milk protein concentrates antigenicity |
