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| 产Iturins族脂肽生防芽孢杆菌的分离、筛选及鉴定 |
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张宝1,2, 于雅琼2, 韩玉竹1, 游成真1, 尚庆茂2, 孔维嘉1, 李平兰1
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| (1.中国农业大学 食品科学与营养工程学院, 北京 100083;2.中国农业科学院 蔬菜花卉研究所, 北京 100081) |
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| 摘要: |
| 为获得能够产Iturins类脂肽的芽孢杆菌以应用于植物的生物防治中,以果蔬体内、根际土壤等多生境中分离的118株细菌为出发菌株,采用平板对峙法、管碟法对其进行了初筛和复筛。结果表明:其中3株菌株对立枯丝核菌、瓜果腐霉和尖孢镰刀菌显示出强烈的抑制作用;通过薄层层析等方法对产Iturins脂肽菌株进行初步分析,最终确定K103为目标菌株。通过形态学鉴定、生理生化试验及16S rDNA的分子生物学等方法,对产抗菌脂肽的K103菌株进行分类学鉴定,最终确定菌株K103为解淀粉芽孢杆菌,并将其命名为解淀粉芽孢杆菌K103(Bacillus amyloliquefaciens K103)。 |
| 关键词: 解淀粉芽孢杆菌 鉴定 脂肽 生物防治 |
| DOI：10.11841/j.issn.1007-4333.2015.05.29 |
| 投稿时间：2014-09-27 |
| 基金项目:公益性行业(农业)科研专项经费项目(201303014);国家自然科学基金项目(31172001) |
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| Isolation, screening and identification of Bacillus spp. with Iturins production |
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ZHANG Bao1,2, YU Ya-qiong2, HAN Yu-zhu1, YOU Cheng-zhen1, SHANG Qing-mao2, KONG Wei-jia1, LI Ping-lan1
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| (1.College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China;2.Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China) |
| Abstract: |
| In order to obtain Bacillus sp.strains producing Iturins for applications in biological control,118 bacterial strains from some vegetable or rhizosphere soil were used in preliminary and secondary screen by the plate confrontation tests and cylinder-plate method.
Among them 3 strains,showing strong antifungal activity against Rhizoctonia solani,Fusarium oxysporum and Pythium aphanidermatum,were analyzed by thin layer chromatography and other methods to detect the ability of the strains to produce Iturins.Finally,the strain K103 was assigned as the target bacterial strain.The organism of interest was identified as a Bacillus amyloliquefaciens strain,and nominated as B.amyloliquefaciens K103 using biochemical,physiological and 16S ribosomal DNA sequencing. |
| Key words: Bacillus amyloliquefaciens identification lipopeptide biological control |
