| 摘要: |
| 为研究不同精粗比全价颗粒饲料对犊牛不同组织中SREBP-1、FASN、SCD1和ApoA1 mRNA表达的影响,选取日龄和体重相近的中国荷斯坦断奶公犊牛12头,分为4组,每组3头,各试验组精粗比分别为75:25(Ⅰ)、70:30(Ⅱ)、65:35(Ⅲ)和60:40(Ⅳ),预试期14d,正试期 56d。结果表明,试验Ⅱ组肝脏、十二指肠和瘤胃中的SREBP-1 和ApoA1 mRNA相对表达量显著高于试验Ⅳ组(P<0.05);试验I组肝脏和十二指肠中的FASN mRNA相对表达量显著高于试验Ⅲ组(P<0.05),而在瘤胃和肌肉中的结果与之相反;试验Ⅰ组犊牛肝脏和十二指肠以及试验Ⅲ组瘤胃中的SCD1 mRNA相对表达量显著高于其他各组(P<0.05)。以上结果说明,日粮高精粗比能够促进犊牛体内脂肪的形成。 |
| 关键词: 荷斯坦牛 基因表达 精粗比 荧光定量PCR |
| DOI：10.11841/j.issn.1007-4333.2015.05.26 |
| 投稿时间：2014-12-26 |
| 基金项目:江苏省科技支撑项目(BE2013387) |
|
| Effects of different concentrate to forage ratio on expression of related fat metabolism enzymes in tissue of calf |
|
ZHAN Jin-shun, GUO Jing, YANG Hong-bo, ZHAN Kang, LIU Hong, LIU Ming-mei, ZHAO Guo-qi
|
| (College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China) |
| Abstract: |
| To study the effects of concentrate to forage ratio in pellet feed on the expression of SREBP-1,FASN,SCD1 and ApoA1 mRNA in different calf tissue.Twelve Holstein bull calves with similar age and weight were divided into 4 groups with 3 calves in each group.Calves in the groups were fed with different concentrate:forage ratios:75:25(Ⅰ),70:30(Ⅱ),65:35(Ⅲ) and 60:40(Ⅳ),respectively.The pre trial period was 14 d and the experimental period was 56 d.The results showed:The expression of related SREBP-1 and ApoA1mRNA in liver,duodenum and rumen of group II was significantly higher than of group Ⅳ.The expression of related FASN mRNA in liver and duodenum of group Ⅰ was significantly higher than that of group Ⅲ,but in rumen and muscle the results obtained were on the contrary.The expression of related SCD1mRNA in liver,duodenum of group Ⅰ and rumen of group Ⅲ was significantly higher than that of other groups.In conclusion,the high concentrate to forage ratio could improve fat synthesis of calf. |
| Key words: Holstein genetic expression concentrate to forage ratio qRT-PCR |
