| 摘要: |
| 为研究固氮芽孢杆菌、固氮巨大芽孢杆菌、解磷假单胞菌、巴西固氮螺菌组成的复合菌剂对小麦根际土壤细菌群落多样性的影响,通过构建16S rRNA克隆文库及采用核糖体DNA扩增片段酶切分析(ARDRA)的方法,以文库库容值(C)、Rarefaction曲线(R)对克隆文库进行评价。系统发育分析表明,对照及菌剂处理样品均检测到酸杆菌门、变形菌门、浮霉菌门、厚壁菌门、拟杆菌门、疣微菌门、放线菌门和芽单胞菌门8个细菌类群,并且优势菌群均为变形菌门和酸杆菌门。接种菌剂后小麦根际土壤检出了绿弯菌门、蓝藻门、产水菌门和硝化螺旋菌门,而梭杆菌门未被检出。假单胞菌属由1.59%增加至21.28%。芽孢杆菌属由未检出增至3.05%。两样品中均未检出固氮螺菌属。多样性分析表明,接种菌剂后,小麦根际土壤细菌多样性指数和丰富度指数均提高。因此,接种菌剂对小麦根际土壤细菌类群的组成及所占比例有较大影响。 |
| 关键词: 菌剂 根际土壤 细菌群落 16SrRNA基因 核糖体DNA扩增片段酶切分析(ARDRA) |
| DOI：10.11841/j.issn.1007-4333.2015.05.11 |
| 投稿时间：2014-12-30 |
| 基金项目:国家高技术研究发展计划863项目(2013AA102802-04) |
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| Influence of nitrogen-fixing, phosphate-solubilizing bacterial agents on bacterial community in wheat rhizosphere soil |
|
HE Guo-qiang1, CHEN San-feng2
|
| (1.Beijing Agricultural Technology Extension Station, Beijing 100029, China;2.College of Biological Sciences/State Key Laboratory of Agricultural Biotechnology, China Agricultural University, Beijing 100193, China) |
| Abstract: |
| The impact of inoculation with bacterial communities (Bacillus sp.,Bacillus megaterium,Pseudomonas alcaligene and Azospirinum brasilense) in rhizospheric soil of wheat was assessed by constructing a 16S rRNA gene clone library,conducting amplified ribosomal DNA restriction analysis (ARDRA),using coverage value (C) and Margalef richness index (R) as evaluated index.Phylogenetic analysis revealed that a total of eight bacterial groups including Acidobacteria,Proteobacteria (including α-, β-, δ-, γ-Proteobacteria),Planctomycetes,Firmicutes,Nitrospirae,Bacteroidetes,Verrucomicrobia and Gemmatimonadetes were existed in both inoculated soil and control soil.The dominant bacterias were Proteobacteria and Acidobacteria in both soil samples.Bacterias belong to Chloroflexi,yanophyta,Aquificae and Nitrospirae excluding Fusobacteria were found in the inoculated treatment.Bacterias belong to Pseudomonas were increased from 1.59% to 21.28%,and bacterias belonging to Bacillus were increased from 0 to 3.05%,while bacterias belong to Azospirillum were not detected in both samples.Diversity analysis showed that soil bacterial Shannon index and Margalef richness index were promoted after inoculation.In conclusion,the inoculation significantly affected the composition of bacterial communities in wheat rhizosphere soil. |
| Key words: bacterial agents rhizosphere soil community composition 16S rRNA genes amplified ribosomal DNA restriction analysis |
