| 摘要: |
| 为探索聚乙二醇(Polyethylene glycol,PEG)引发种子对高粱芽苗耐水分亏缺的生理调节效应,通过盆栽土培试验,将高粱种子(辽粘6号)采用20%的PEG 8000溶液,在18℃条件下浸泡48h,设置土壤100%(CK)、85%、70%、55%和40%供水量模拟水分亏缺,研究种子萌发、相对含水量、叶绿素、根系活力、抗氧化系统、渗透调节系统和根系解剖结构等的适应特点与调节方式。结果表明:1)PEG引发种子可促进水分亏缺下种子的萌发、增强种子的活力和促进出苗;2)PEG引发种子对幼苗地下部的作用效果大于地上部,在土壤水分亏缺不超过70%供水(Soil water content,SWC-70)时叶绿素降解和根系活力降低幅度较小;3)PEG引发种子后抗氧化系统和渗透调节系统均发生较大变化,POD和O2-是维持水分亏缺的抗氧化系统关键因子,可溶性糖和质膜透性的调节是提高幼苗机体抗性的重要因素;4)种子引发对根系后生木质部和后生韧皮部数量和体积的调节是其适应水分亏缺的重要方式。土壤水分亏缺条件下合理应用聚乙二醇(PEG)引发种子可以有效提高高粱芽苗期的抗旱能力。 |
| 关键词: 高粱 芽苗 聚乙二醇 水分亏缺 生理调节 |
| DOI：10.11841/j.issn.1007-4333.2015.05.06 |
| 投稿时间：2014-12-20 |
| 基金项目:国家科技支撑计划(2014BAD07B02);辽宁省农业青年科技人才培养计划(2014019) |
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| Study physiological process of sorghum (Sorghum bicolor L.) response to drought after treatment with polyethylene glycol |
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ZHANG Fei, WANG Yan-qiu, ZHU Kai, DUAN You-hou, KE Fu-lai, ZOU Jian-qiu
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| (Innovation Center, Liaoning Academy of Agricultural Sciences, Shenyang 110161, China) |
| Abstract: |
| To investigate physiological regulation effect on plant drought tolerance by treating sorghum (Sorghum bicolor L.) seeds with polyethylene glycol (PEG).The sorghum hybrid (cv.Liao waxy No.6) seeds were pretreated with PEG 8000 solution (20% concentration) for 48 h at 18℃ before sowing in soil.To simulate drought environment,seedlings were provided with 100 (CK),85,70,55 and 40 percentage water supply respectively.Seed germination rate,leaf and root relative water content,chlorophyll content,root activity,antioxidant systems,osmotic adjustment system and root anatomical structure were all investigated.Results showed that:1) PEG pretreatment promoted seed germination,improved seed emergence and viable rate under soil drought condition;2) When water supply was under 70% (Soil water content,SWC-70),both chlorophyll degradation and root activity were decreased;3) Antioxidant system and osmotic adjustment system changed prominently after PEG treatment.POD and O2- were two critical factors for antioxidant system.Soluble sugar and membrane permeability were important factors in osmotic regulation to improve seedling drought tolerance;4) The number and volume of xylem and phloem played important role in plant adapting to drought.Appropriate PEG treatment of sorghum seeds could improve drought tolerance at seeding phase. |
| Key words: sorghum seed polyethylene glycol water deficit physiology regulation |
