| 摘要: |
| 为研究运输应激对脑内五羟色胺(5-hydroxytryptamine,5-HT)表达的影响及酸枣仁散对运输应激的调控作用,试验采用摇床模拟公路运输途中的摇晃、高温和拥挤等因素,建立大鼠运输应激模型(35 ℃,60 r/min,每天应激2 h,连续7 d)。将40只大鼠随机分为对照组、酸枣仁组、应激组和应激＋酸枣仁组,分别处理7 d后,断头取脑,利用酶联免疫吸附法(ELISA)和免疫组化法检测各组大鼠脑区5-HT含量变化及分布。酶联免疫吸附法检测结果显示,与对照组相比,应激组大鼠中缝核、海马和纹状体等脑区5-HT表达量均明显减少(P<0.05),应激＋酸枣仁组较应激组5-HT表达量显著增加(P<0.05)。免疫组化结果显示,在中缝背核及其亚核DRDsh、DRv和DRc亚核,应激组大鼠5-HT灰度值与对照组相比显著下降(P<0.05),应激＋酸枣仁组与应激组相比显著上升(P<0.05)。以上结果表明,运输应激可以导致中枢神经系统部分脑区5-HT含量下降,而酸枣仁散可能通过上调脑内5-HT的含量来调控运输应激。 |
| 关键词: 酸枣仁散 大鼠 应激 5-HT 脑 中缝背核 |
| DOI：10.11841/j.issn.1007-4333.2015.04.21 |
| 投稿时间：2014-11-20 |
| 基金项目:"十二五"国家科技支撑计划项目(2011BAD34B01);公益性行业(农业)科研专项(201003060-10);中央高校基本科研业务费专项资金(2011JS008) |
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| Effect of Suanzaoren San on 5-HT expression in the brain of transport stressed rats |
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WANG Li-li1, MA Tong-hui1, YU Ming-yuan1, TANG Lin-jie1, ZHANG Wen-jun1, XU Jian-qin1, LIU Feng-hua2, MA Yun-fei1, TENG Ke-dao1
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| (1.College of Veterinary Medicine, China Agricultural University, Beijing 100193, China;2.College of Animal Science and Technology, Beijing University of Agriculture, Beijing 102206, China) |
| Abstract: |
| In order to investigate the impact of transport stress on 5-HT in rat brain and the regulation of Suanzaoren San (named as SZR), a swing bed was used as transport stress module (named as TS) under condition of 35 ℃, 60 rpm and 2 h/d for 7 days.Forty rats were divided randomly into four groups:the control group, the SZR group, the TS group and the SZR+TS group.The brain tissues were taken after 7 days treatment.The level of 5-HT in different regions of the brain were detected using enzyme-linked immunosorbent assay (ELISA).The distribution and quantity of serotonergic neurons were also examined by immunohistochemical technique.The results of ELISA showed that the content of 5-HT was significantly decreased (P<0.05) in dorsal raphe nucleus, hippocampus and striatum after transport stress.Moreover, 5-HT content in the SZR+TS group was significantly increased (P<0.05) in these regions, compared with the TS group.The immunohistochemical results showed that 5-HT expression in the TS group was decreased significantly compared to control group in DRDsh, DRv and DRc (P<0.05).While the level of 5-HT in the TS+SZT group was increased compared with the TS group.In conclusion, transport stress can decrease the level of 5-HT in different brain regions, and SZR could regulate transport stress by increasing the level of 5-HT. |
| Key words: SZR rat stress 5-HT brain dorsal raphe nucleus |
