| 摘要: |
| 利用扫描电子显微镜对小麦胚乳发育过程中淀粉粒的形态和成熟胚乳中的A、B型淀粉粒进行观察。电子显微镜扫描结果显示,花后5~10 d,糯小麦胚乳中可见到一些体积较小的A型淀粉粒,10~15 d以A型淀粉粒数量增多和体积增大为主;B型淀粉粒在花后15 d已经形成,但此时B型淀粉粒的数量少;花后20 d—成熟前B型淀粉粒的数量和体积逐渐增长。糯小麦成熟籽粒胚乳中淀粉粒的直径分布为0.96~34.92 μm。与普通小麦相比,糯小麦A型淀粉粒的形状较扁平;表面没有普通小麦光滑;B型淀粉粒的形状比普通小麦更无规则,且更容易发生聚集现象。另外,发现在A型淀粉粒上有许多孔洞;在5 000倍数下观察,发现这些孔洞结构从A型淀粉粒表面一直延伸到其内部。 |
| 关键词: 糯小麦 淀粉 淀粉粒 扫描电子显微镜 显微观察 |
| DOI：10.11841/j.issn.1007-4333.2015.04.04 |
| 投稿时间：2014-10-29 |
| 基金项目:国家自然科学基金(31371615);安徽省高等学校省级自然科学研究项目(KJ2012Z105) |
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| Microscopic study of morphological changes of A and B type starch grains during the development of endosperm in waxy wheat |
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LU Ping, CHEN Jun, WU Yuan-yuan, ZHOU Jian, ZHENG Wen-yin, ZHANG Wen-ming, YAO Da-nian
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| (College of Agronomy/Key Laboratory of Wheat Biology and Genetic Breeding in Huang-huai Southern Areas of Ministry of Agriculture/Collaborative Innovation Center on Crops of Anhui Province, Anhui Agricultural University, Hefei 230036, China) |
| Abstract: |
| Scanning electron microscope(SEM) was used to investigate the morphologic changes of starch granule in developing endosperm and to observe A and B-type starch granules in mature wheat endosperm.SEM results showed that many small A-type starch were visible in waxy wheat endosperm, during 5-10 days after flowering, followed by an increase on starch granule size during 10-15 days, B-type starch granules were formed 15 days after pollination, but only a few B-type starch granules were detected at this point;The number and size of B-type starch granule increased gradually from the 20th day after pollination to maturity.The diameter of starch granules in mature grain endosperm of waxy wheat ranged from 0.96 μm to 34.92 μm.Compared with the common wheat, A-type starch granules of the waxy wheat were flatter and the surface of A-type starch granules was less smooth.The shapes of B-type starch granules were more irregular than that of common wheat and the granules were more often aggregated.Further studies showed that there were many holes on A-type starch granules, under 5 000 fold magnification, and the holes were extended from surface to its interior. |
| Key words: waxy wheat starch starch granules scanning electron microscopy (SEM) microscopic examination |
