| 摘要: |
| 为了解山西小麦品质现状并且为今后山西小麦育种提供材料和依据,利用十二烷基硫酸钠聚丙烯酰胺凝胶电泳(Sodium dodecyl sulfate polyacrylamide gel electrophoresis,SDS-PAGE)方法分析123份山西小麦品种资源高分子量谷蛋白亚基(High molecular weight glutenin subunits,HMW-GS)的组成。结果表明:在123份供试材料中,共检测出18种HMW-GS,其中Glu-A1位点上有1、2*和Null共3种,Glu-B1位点上有7+8、7+9、7、6+8、17+18、14+15、20、13+16和13+19共9种,Glu-D1位点上有2+12、5+10、5+12、2+10、3+12和4+12共6种;亚基Null、7+8和2+12在各自位点上出现频率最高,分别为78.05%、60.16%和65.85%。亚基组合类型共34种,主要是Null/7+8/2+12,占45.53%,其次是Null/7+9/2+12,占12.20%,优良亚基组合类型1/7+8/5+10与1/17+18/5+10相当缺乏。育成品种与地方品种比较分析发现,在Glu-A1位点上优良亚基1提高14.16%;Glu-B1位点上7+8下降9.05%;Glu-D1位点上5+10在地方品种中没有出现,但在育成品种中达14.89%。同时筛选出10份得9和10分的优质品种资源,还有12份含稀有亚基的品种资源,可作为山西今后小麦品质育种的亲本材料。 |
| 关键词: 小麦 地方品种 育成品种 外引品种 高分子量谷蛋白亚基 |
| DOI：10.11841/j.issn.1007-4333.2015.04.03 |
| 投稿时间：2014-08-03 |
| 基金项目:山西省人才引进与开发专项(2011);山西省自然科学基金(2014011004-3);山西农业大学科技创新基金项目(201225) |
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| Analysis on components of HMW-GS in Shanxi wheat cultivars(lines) from different sources |
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CHEN Wei-guo, WANG Shu-guang, SHI Yu-gang, SUN Dai-zhen
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| (Shanxi Agricultural University, Taigu 030801, China) |
| Abstract: |
| In order to know present situation of wheat quality and to provide materials and basis for the future wheat quality improvement in Shanxi, high molecular weight glutenin subunits(HMW-GS) composition of 123 wheat germplasm resources were analyzed by means of sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE).The results were as follows: There were 18 alleles at Glu-1 locus, including 3 (1, 2* and Null) at Glu-A1, 9 (7+8, 7+9, 7, 6+8, 17+18, 14+15, 20, 13+16 and 13+19) at Glu-B1, 6 (2+12, 5+10, 5+12, 2+10, 3+12 and 4+12) at Glu-D1, and the frequency of Null, 7+8 and 2+12 were the highest at each of loci, reaching 78.05%, 60.16% and 65.85%, respectively.There are 34 subunit composition types, the predominant was Null/7+8/2+12 with a frequency of 45.53%, and the second was Null/7+9/2+12 with a frequency of 12.20%.However, the types of good subunit composition 1/7+8/5+10 and 1/17+18/5+10 were rare.Comparative analysis found that elite subunit 1 coded at Glu-A1 in bred varieties increased by 14.16% in landraces, and the 7+8 coded at Glu-B1 fell by 9.05%.The frequencies of 5+10 coded at Glu-D1 were decreased 14.89% in bred varieties, while zero in landraces.10 elite resources scored 9 or 10 and 12 with rare subunits were screened, and they could be used as parent materials for future wheat breeding in Shanxi. |
| Key words: wheat local variety bred variety introduced variety high molecular weight glutenin subunits (HMW-GS) |
