| 摘要: |
| 为确立治疗致病性大肠杆菌病的蒙药组方,采用牛津杯法,选取15味蒙药对致病性大肠杆菌进行体外抑菌试验,筛选O1血清型致病性大肠杆菌的敏感蒙药.结果表明,O1血清型致病性大肠杆菌对12味蒙药敏感.并通过棋盘法测定部分抑菌浓度,筛选出5组6味蒙药,结合体外抑菌试验,通过L8(27)正交试验设计,筛选出最佳体外抑菌蒙药组合,再通过L9(34)正交试验设计进行剂量优化.结果显示,结合正交设计和体外抑菌试验确立了5组蒙药组方,且其抑菌效果显著优于各单味蒙药的抑菌效果,即5组蒙药复方中最小抑菌圈为30.49 mm,而单味蒙药最大抑菌圈为24.42 mm. |
| 关键词: 蒙药组方 大肠杆菌 体外抑菌试验 正交设计 |
| DOI：10.11841/j.issn.1007-4333.2015.03.21 |
| 投稿时间：2014-08-18 |
| 基金项目:国家自然科学基金资助项目(31060318,31260570); "十二五"国家科技支撑项目(2012BAD12B09-3) |
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| Selection of the compound Mongolian medicine on Escherichia coli inhibition by bacteriostasis experiment in vitro |
|
YANG Si-qin1, AO-ri-ge-le1, WANG Chun-jie2, Man-da1, Si-mu-ji-de1, LIU Wen-cai1
|
| (1.College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China;2.College of Veterinary, Inner Mongolia Agricultural University, Hohhot 010018, China) |
| Abstract: |
| In order to establish Mongolian medicine prescription treating Escherichia coli disease, this study used the Oxford Cup method and selected 15 kinds of Mongolian medicines carried out by vitro test of inhibitory bacteria and then screened prescriptions which are sensitive to O1 serological type of pathogenic Escherichia coli.The results showed that,the serological types of pathogenic Escherichia coli is sensitive to 12 kinds of medicines.Using checkerboard method combined with bacteriostasis experiment in vitro,this study measured fractional inhibitory concentration of five groups of six flavour medicines and selected optimum medicine combination in vivo utilizing L8(27)orthogonal test and followed by L9(34) orthogonal test to optimize dose.Combining the orthogonal test and vitro test,this study determined 5 groups of presicriptions,the bacteria inhibiting effect of which is obviously better than that single-flavor. |
| Key words: prescriptions of Mongolian medicines Escherichia coli vitro test of inhibitory bacterial orthogonal design |
