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| 转基因玉米NK603品系成分LAMP快速PCR检测方法的建立 |
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甄贞, 张明辉, 刘营, 敖金霞, 于艳波, 李璐, 曲波, 仇有文, 高学军, 张克俭
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| (东北农业大学 生命科学学院/东北农业大学农业生物功能基因重点实验室/农业部转基因生物产品成分监督检验测试中心(哈尔滨), 哈尔滨 150030) |
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| 摘要: |
| 根据转基因玉米品系NK603外源插入片段与玉米基因组序列设计品系特异性引物,建立转基因玉米品系NK603的品系特异性环介导等温扩增(Loop-mediated isothermal amplification,LAMP)检测方法,并对此方法进行灵敏度、特异性测试.试验表明该方法能够快速检测出转基因玉米NK603品系成分,检测灵敏度为0.1%,并具有较高的特异性和较好的稳定性. |
| 关键词: 转基因玉米NK603品系 环介导等温扩增 快速检测 |
| DOI：10.11841/j.issn.1007-4333.2015.03.04 |
| 投稿时间：2014-10-19 |
| 基金项目:转基因重大专项资助项目(2014zx08004-002); 国家基础科学人才培养基金(J1210069); 东北农业大学博士启动基金项目(2012RCB19) |
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| Development of loop-mediated isothermal amplification(LAMP) method for rapid detection of transgenic maize NK603 |
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ZHEN Zhen, ZHANG Ming-hui, LIU Ying, AO Jin-xia, YU Yan-bo, LI Lu, QU Bo, QIU You-wen, GAO Xue-jun, ZHANG Ke-jian
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| (College of Life Sciences/Agricultural Biological Functional Gene Laboratory/Supervision and Test Center(Harbin) for MolecularCharacteristics of Genetically Modified Plants of Ministry of Agriculture, Northeast Agricultural University, Harbin 150030, China) |
| Abstract: |
| According to both NK603 exogenous insert sequences and the maize plant genome sequences,specific primers were designed.A loop-mediated isothermal amplification (LAMP) method for transgenic maize NK603 was established.The specificity sensitivity and stability of the LAMP method were investigated.The results showed that this method could specifically detect transgenic maize NK603 component rapidly with limit of detection (LOD) as low as 0.1%,and had a good specificity and stability. |
| Key words: transgenic maize NK603 loop-mediated isothermal amplification (LAMP) rapid detection |
