| 摘要: |
| 采用相关序列扩增多态性(Sequence-related amplified polymorphism,SRAP)分子标记方法,对收集到的来源于11个国家的19个剑豆种群进行遗传多样性分析。结果表明:1)在扩增出的274条清晰条带中,具有多态性的有144条,占总数的52.6%;2)28对引物的多态性指数(Polymorphism information content,PIC)介于0.16~0.43,平均值为0.28;3)剑豆总遗传多样性指数(Ht)为0.285 6,基因流(Nm)为0.065 6;4)通过分子方差分析(Analysis of molecular variance,AMOVA)发现,剑豆种群间差异占总差异的88%,种群内差异占总差异的12%,种群间差异为剑豆差异的主要来源;5)主坐标分析(Principal coordinates analysis,PCoA)和基于遗传距离的聚类分析表明,19个剑豆种群可分为5个类群,每个类群均有不同的表型。 |
| 关键词: 剑豆 相关序列扩增多态性 遗传多样性 |
| DOI：10.11841/j.issn.1007-4333.2015.02.008 |
| 投稿时间：2014-05-31 |
| 基金项目:广州市科技计划项目(2010Z1-E241); 农业部"948"项目(2011-Z50) |
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| Genetic diversity of Canavalia ensiformis (L.)DC. accessions revealed by SRAP markers |
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LIU Ming-qian1, CHEN Li-jun2, DING Mei-mei1, OUYANG Kun-xi1, HUI Wen-kai1, LI Jun-cheng1, CHEN Xiao-yang1
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| (1.College of Forestry/Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, South China Agricultural University, Guangzhou 510642, China;2.Center for Teaching & Research Base, South China Agricultural University, Guangzhou 510642, China) |
| Abstract: |
| Canavalia ensiformis is an under-exploited legume that has been used as forage, green manure and cover crop.It can potentially be used in numerous other ways, including nutraceuticals and phytopharmaceuticals.In this study, sequence-related amplified polymorphism (SRAP) markers were used to assess the genetic diversity and relationship of 19 C.ensiformis accessions originated from 11 countries.The results showed that:1) In total, 274 clear bands were amplified and 144 fragments (52.6%) were polymorphic;2) The polymorphism information content (PIC) values ranged from 0.16 to 0.43, with an average of 0.28;3) The total genetic diversity (Ht) was 0.285 6, the estimate of gene flow (Nm) was 0.065 6;4) The results from the analysis of molecular variance (AMOVA) revealed that the most significant variation, 88% of the total variation, occurred among accessions and 12% was attributed to within accession variation;5) The cluster analysis and principal coordinates analysis (PCoA) produced similar results, whereby the 19 C.ensiformis were divided into five clusters, each cluster was composed of different accessions and had different phenotypic traits.This study provided the theoretical basis for biodiversity studies and breeding programs of Canavalia ensiformis. |
| Key words: Canavalia ensiformis sequence-related amplified polymorphism(SRAP) genetic diversity |
