| 摘要: |
| 为研究蒙药对牛源致病性大肠杆菌的体外抑菌效果,采用牛津杯法测定15种蒙药的抑菌圈直径,结果表明,其中朝您·哈日莫各和扫龙·吉木斯两味蒙药对5种血清型牛源致病性大肠杆菌抑菌效果较好,达到极敏和高敏;通过微量稀释法和平板法测定最小抑菌浓度(MIC)和最低杀菌浓度(MBC),结果显示,朱如拉、朝您·哈日莫各、扫龙·吉木斯、浑钦、高勒图·宝日、给喜古讷和阿如拉等7种蒙药对5种血清型牛源致病性大肠杆菌综合抑菌效果较好,其MIC为0.063~0.500 g/mL,MBC为0.125~0.500 g/mL。 |
| 关键词: 蒙药 血清型 致病性大肠杆菌 抑菌作用 |
| DOI：10.11841/j.issn.1007-4333.2015.01.017 |
| 投稿时间：2014-05-05 |
| 基金项目:国家自然科学基金资助项目(31060318,31260570); 国家科技支撑项目(20BAD12B09-02); "十二五"国家科技支撑项目(2012BAD12B09-3) |
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| Antibacterial effects of Mongolian medicines on bovine pathogenic Escherichia coli in vitro |
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YANG Si-qin1, AO-ri-ge-le1, WANG Chun-jie2, SI-mu-ji-de1, BAO Yong-guang3
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| (1.College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China;2.College of Veterinary, Inner Mongolia Agricultural University, Hohhot 010018, China;3.Inner Mongolia Gao Lin Tun Breeding Farm, Tongliao 028037, China) |
| Abstract: |
| The purpose of this paper is to study the effects of Mongolian medicines on the antibacterial effect of bovine pathogenic Escherichia coli.The diameter of inhibition zone was determined by Oxford cup method.The Oxford cup test results showed that among 15 Mongolian medicines,the two which had a better inhibitory effect on 5 different serotypes of pathogenic E. coli were chaonin Harimog and Solong Gims.Minimal inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were investigated by the micro dilution method and plate method.The Micro dilution method explained that 7 kinds of Mongolian medicines that had better inhibitory effects on different serotypes from bovine Pathogenic E.coli were Zhu Rula,chaonin Harimog,Solong Gims,Hun Qin,Gaoletu · Baori,Geixigune and Arule with 0.063-0.500 g/mL of MIC and 0.125-0.500 g/mL of MBC. |
| Key words: Mongolian medicines serotypes Pathogenic Escherichia coli bacteriostasis |
