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| 短柄草Hsf家族全基因组鉴定、分类和高温响应 |
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李盼松1,2, 郑炜君1, 周永斌1, 陈明2, 柴守诚1, 马有志2, 马翎健1, 徐兆师2
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| (1.西北农林科技大学 农学院, 陕西 杨凌 712100;2.中国农业科学院 作物科学研究所/国家农作物基因资源与基因改良重大科学工程/农业部麦类生物学与遗传育种重点实验室, 北京 100081) |
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| 摘要: |
| 分析短柄草Hsf家族成员的进化关系,并进行分类;利用多种软件和在线工具分析短柄草Hsf的蛋白结构功能域和基因启动子区域的顺式作用元件;分析基因组信息,进行基因的染色体定位;利用荧光实时定量技术(qRT-PCR),分析短柄草Hsf基因在高温胁迫下的表达模式,鉴定出一些高温诱导表达的Hsf基因。 |
| 关键词: 短柄草 热激因子 全基因组鉴定 高温 表达模式 |
| DOI：10.11841/j.issn.1007-4333.2015.01.002 |
| 投稿时间：2014-02-26 |
| 基金项目:转基因生物新品种培育科技重大专项(2014ZX08002-002,2014ZX08002-003B) |
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| Genome-wide identification,classification and high temperature responses of Hsf family in Brachypodium distachyon |
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LI Pan-song1,2, ZHENG Wei-jun1, ZHOU Yong-bin1, CHEN Ming2, CHAI Shou-cheng1, MA You-zhi2, MA Ling-jian1, XU Zhao-shi2
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| (1.College of Agronomy, Northwest A&F University, Yangling 712100, China;2.Institute of Crop Science/National Key Facility for Crop Gene Resources and Genetic Improvement/Key Laboratory of Biology and Genetic Improvement of Triticeae Crops of Ministry of Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100081, China) |
| Abstract: |
| High temperature stress is one of the most important abiotic stress factors that affect the crop yield and quality.Heat shock response is mainly regulated by heat shock transcription factor (Hsf).Softwares and online tools were used to analyze the Hsf gene structures,protein function domains,chromosomal locations,phylogenetic relationships and cis-elements.Twenty-four Hsf genes were systematically identified,classified into 13 subclasses according to their phylogenetic relationships and located on 5 chromosomes.Each of the Hsf genes contained introns and each contained cis-elements in their promoter regions which might participate in drought,low temperature,high temperature,high light and ABA stress responses.Quantitative real-time PCR (qRT-PCR) was used to analyze the expressions of the Hsf genes under high temperature stress and most of the Hsf genes showed up-regulated.In this work,identification,chromosomal location,classification,genetic structure,protein structure and expression analysis of Brachypodium distachyon Hsf genes under high temperature condition would provide the theory basis for the further research on the functions of Hsf genes. |
| Key words: Brachypodium distachyon Hsf genome-wide identification high temperature express pattern |
