| 摘要: |
| 为探明低温对甘蔗根系的影响, 本研究采取水培试验的方法, 选用不抗寒品种ROC22和抗寒品种GT28的完整根系为供试材料, 测定常温(25 ℃)与低温(4 ℃)条件下甘蔗幼苗根系的根长、根体积、根细胞排列、根系活力、丙二醛(MDA)、脯氨酸、可溶性糖与可溶性蛋白质含量以及过氧化物酶(POD)和超氧化物歧化酶(SOD)活性相关指标。结果表明:在低温胁迫下, GT28根长、根体积均大于ROC22, 但都显著低于常温生长;低温胁迫3 d后, 根系细胞膨大变形, 细胞结构散乱, 以ROC22更为明显;随着低温时间的延长, 2个品种根系活力均显著降低, 而ROC22的降幅更大;在低温持续胁迫下, 根系MDA、脯氨酸、可溶性糖、可溶性蛋白含量以及POD和SOD活性等指标均高于常温生长, 呈先上升后降低趋势, 抗寒品种GT28的可溶性糖含量、脯氨酸含量、POD和SOD酶活性均高于不抗寒品种ROC22。结论:在低温胁迫下根系生长和生理生化代谢的差异是不同品种抗寒能力的生理基础。 |
| 关键词: 甘蔗 根 低温胁迫 |
| DOI：10.11841/j.issn.1007-4333.2014.06.10 |
| 投稿时间：2014-02-28 |
| 基金项目:广西自然科学基金创新团队项目(2011GXNSFF018002);广西八桂学者和特聘专家专项经费;广西科学研究与技术开发计划项目(桂科产1123008-1);广西科技基础条件平台(重点实验室)建设项目(11-031-31);广西农科院团队项目(桂农科2011YT01) |
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| Response of chilling stress on root morphology and protection systems of sugarcane seedlings |
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SUN Bo1, LIU Guang-ling1, YANG Li-tao1,2, LI Yang-rui1,2
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| (1.Agricultural College/State Key Laboratory of Conservation and Utilization of SubtropicalAgro-bioresources, Guangxi University, Nanning 530005, China;2.Sugarcane Research Center/Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture/Guangxi Crop Genetic Improvement and Biotechnology Laboratory/Guangxi Key Laboratory ofSugarcane Genetic Improvement, Chinese Academy of Agricultural Sciences, Nanning 530007, China) |
| Abstract: |
| This experiment aimed to study the effect of low temperature on roots growth of different sugarcane varieties with varied cold resistance in order to provide theoretical references for future relevant researches.The complete roots of the young plants derived from water culture for both cold resistant sugarcane variety GT28 and the cold sensitive sugarcane variety ROC22 were used as the plant materials.The plants of the both varieties were treated with normal room temperature (25 ℃, control) and low temperature (4 ℃), respectively.Various parameters, including root length, root volume, root micro-structure, root vigor activities, malonaldehyde (MDA), contents of proline, soluble sugar, soluble protein, peroxidase (POD) and superoxide dismutase (SOD) were measured.As the cold stress duration prolonged, the average root length and root volume were decreased by the cold stress compared to the normal temperature, but the decreases were less in GT28 than in ROC22.After 3 days of cold stress, the root micro-structure were getting swelling and deformating, and disorder of cellular structures were observed in the roots of both tested varieties, but the deformation was much more significant in ROC22 than in GT28.Under the prolonged cold stress, the root activity was significantly weakened for both sugarcane varieties, but it was more serious for the variety ROC22.The contents of MDA, proline, soluble sugar, soluble protein, and the activities of POD and SOD in the roots were higher than those in the control and the patten showed a progressive increasing and then decreasing.The contents of soluble sugar and proline and the activities of POD and SOD in cold resistant variety of GT28 were higher than those of cold susceptible variety of ROC22.The present results indicate that cold resistnce/suscepitibility is variety-dependence based on, root growth and metabolism of physiology and biochemistry. |
| Key words: sugarcane roots cold stress |
