| 本文已被：浏览 972次 下载 736次 |
码上扫一扫！ |
| 小麦品种Bogatka抗白粉病基因的分子标记定位 |
|
张晶, 彭福祥, 刘祜宇, 耿妙苗, 董宏图, 李映辉, 刘婉辉, 刘鑫, 李峰, 解超杰, 孙其信
|
|
|
| (中国农业大学 农学与生物技术学院/农业生物技术国家重点实验室/农业部作物基因组学与遗传改良重点开放实验室/北京市作物遗传改良重点实验室/教育部作物杂种优势研究与利用重点实验室, 北京 100193) |
|
| 摘要: |
| 为明确小麦品种Bogatka抗白粉病性状的遗传基础, 利用感病亲本薛早和Bogatka以及其杂交所得"薛早/Bogatka"F1与薛早回交得到的BC1群体, 进行遗传分析和分子标记定位。结果表明, Bogatka中含有1个显性抗白粉病基因, 暂命名为MlBogatka。进一步利用BSA法对BC1分离群体进行分子标记检测, 得到与MlBogatka基因连锁的分子标记STSBCD135、Xgwm501和Xwmc332, 并构建遗传连锁图。根据这些分子标记的染色体定位信息, 该基因位于小麦2B染色体长臂。综合对该基因的标记定位和Pm6基因特异分子标记检测结果, 推测该基因可能是Pm6或与Pm6位点紧密连锁的抗白粉病基因。本研究结果为Bogatka在小麦抗白粉病育种中的利用提供了依据。 |
| 关键词: 普通小麦 遗传分析 Bogatka 抗白粉病基因 分子标记 |
| DOI：10.11841/j.issn.1007-4333.2014.06.02 |
| 投稿时间：2014-03-24 |
| 基金项目:国家自然科学基金项目(31271708);国家"973"计划项目(2009CB118300) |
|
| Molecular mapping of the powdery mildew resistance gene in wheat cultivar Bogatka |
|
ZHANG Jing, PENG Fu-xiang, LIU Hu-yu, GENG Miao-miao, DONG Hong-tu, LI Ying-hui, LIU Wan-hui, LIU Xin, LI Feng, XIE Chao-jie, SUN Qi-xin
|
| (College of Agronomy and Biotechnology/State Key Laboratory of Agri-Biotechnology/Key Laboratory of Crop Genomics and Genetic Improvement, Ministry of Agriculture/Beijing Key Laboratory of Crop Genetic Improvement/Key Laboratory of Crop Heterosis Research & Utilization, Ministry of Education, China Agricultural University, Beijing 100193, China) |
| Abstract: |
| Bogatka, a Polish elite wheat cultivar, is highly resistance to powdery mildew in Beijing.In order to clarify the genetics basis of the resistance, a BC1 segregating population was acquired from Xuezao/Bogatka//Xuezao, and was inoculated with Blumeria graminis f.sp.tritici(Bgt) isolate E09.Results demonstrated that the powdery mildew resistance of Bogatka was conferred by a dominant gene, temporally designated MlBogatka.Bulked segregant analysis was used to detect the molecular markers linked to MlBogatka.Two polymorphic SSR markers (Xgwm501, Xwmc332) and one STS marker STSBCD135 on chromosome 2BL were linked to the resistance gene.A genetic map of MlBogatka was established with these markers.Based on the map location and the existence of the Pm6 specific STS marker, we speculated that this gene was likely to be Pm6 or other gene which was closely linked to Pm6 locus.This result provides valuable information for the utilization of Bogatka in wheat powdery mildew resistance breeding program. |
| Key words: common wheat genetic analysis Bogatka powdery mildew resistance gene molecular mapping |
