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| 鲟鱼硫酸软骨素制备工艺优化 |
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宋居易1, 桂萌1, 马长伟1, 王顺2, 彭朝辉3, 李平兰1
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| (1.中国农业大学 食品科学与营养工程学院, 北京 100083;2.北京市水科学技术研究院, 北京 100048;3.北京北水食品工业有限公司, 北京 101108) |
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| 摘要: |
| 为制备高纯度鲟鱼硫酸软骨素，利用鲟鱼头骨和脊骨为原料，经过硫酸软骨素提取专用酶与碱性蛋白酶联合酶解新工艺，并进一步经季铵盐纯化、乙醇沉淀，制得硫酸软骨素纯品。硫酸软骨素提取专用酶和碱性蛋白酶提取的最佳工艺为：硫酸软骨素提取专用酶用量15 mg/g，酶解温度55 ℃，酶解时间4 h；碱性蛋白酶用量50 mg/g，酶解温度60 ℃，酶解时间2 h。季铵盐-乙醇沉淀法纯化最佳工艺为：季铵盐质量浓度1 g/100 mL、作用温度 20 ℃，作用时间1.5 h；乙醇沉淀体积分数为75%、溶液pH为7。该工艺制得的鲟鱼头骨、脊骨硫酸软骨素纯品的产率分别为19.5%、22.0%，纯度分别为94.80%、95.32%，蛋白含量分别为0.287%、0.267%。 |
| 关键词: 鲟鱼 头骨 脊骨 硫酸软骨素 提取 纯化 |
| DOI：10.11841/j.issn.1007-4333.2014.05.16 |
| 投稿时间：2013-12-19 |
| 基金项目:北京市鲟鱼、鲑鳟鱼创新团队项目（SCGWZJ 20121105-2） |
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| Optimized technology of chondroitin sulfate preparation from cultured sturgeon |
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SONG Ju-yi1, GUI Meng1, MA Chang-wei1, WANG Shun2, PENG Chao-hui3, LI Ping-lan1
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| (1.College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China;2.Beijing Water Science and Technology Institute, Beijing 100048, China;3.Beijing North Water Food Industry Limited Company, Beijing 101108, China) |
| Abstract: |
| Research aimed to prepare high pure production of chondroitin sulfate of sturgeon.The pure chondroitin sulfate from skull and spinal of cultured sturgeon was extracted by sequential hydrolysis with the chondroitin sulfate extraction of special enzyme and alkaline proteinase and purified by quaternary ammonium salt and ethanol.The optimum enzymolysis procedure by the chondroitin sulfate extraction of special enzyme and alkaline proteinase was as follows:15 mg/g chondroitin sulfate extraction of special enzyme was added and kept at 55 ℃ for 4 h for hydrolysis, and then 50 mg/g alkaline proteinase was added and kept at 60 ℃ for 2 h.The optimum procedure of method of quaternary ammonium salt and ethanol precipitation was as follows:1 g/100 mL quaternary ammonium salt solution was added and kept at 20 ℃ for 1.5 h and ethanol solution adjusted to pH 7 was 75%.Under these conditions, the yield of the pure products of chondroitin sulfate from skull and spinal was 19.5% and 22.0%, the purity was 94.80% and 95.32%, and the protein level was 0.287% and 0.267%, respectively. |
| Key words: cultured sturgeon skull spinal chondroitin sulfate extraction purification |
