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基施硒肥对蚕豆籽粒硒含量、营养成分及抗氧化性的影响
张华华1, 李放1, 李航宇1, 葛军勇1, 徐东旭2, 康玉凡1
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(1.中国农业大学 农学与生物技术学院, 北京 100193;2.张家口市农业科学院, 河北 张家口 075000)
摘要:
为探明硒对蚕豆籽粒产量、硒含量、营养物质含量及抗氧化性的影响,以“崇礼蚕豆”和“凤豆6号”2个品种为试验材料,采用盆栽试验研究5个土壤施硒水平(Na2SeO3,以Se计0、3.1、6.2、12.4和24.8 mg/kg)对硒在蚕豆籽粒中的富集规律、营养物质含量及1,1-二苯基-2-三硝基苯肼(DPPH)自由基清除力的影响。结果表明:在本试验条件下,适宜硒浓度能够提高蚕豆籽粒产量,而高浓度硒则降低蚕豆产量;施硒能够显著提高蚕豆籽粒硒含量(崇礼蚕豆和凤豆6号分别较对照提高21.3~35.8和4.97~7.60倍),籽粒硒含量与施硒浓度之间呈对数关系;硒同时可以影响籽粒中粗蛋白、粗纤维、粗灰分和单宁等物质的含量,而对总碳水化合物及植酸含量的影响并不显著;施硒可以显著提高籽粒提取物DPPH自由基清除力;硒对籽粒中物质含量和抗氧化性的影响在蚕豆品种间也存在一定的差异。因此,合理施硒具有增加硒含量和改善蚕豆营养品质的双重效益,低浓度硒处理(3.1 mg/kg)便适于富硒蚕豆的开发。
关键词:    蚕豆  营养物质  抗营养物质  DPPH自由基清除力
DOI:10.11841/j.issn.1007-4333.2014.05.09
投稿时间:2013-09-02
基金项目:国家现代农业产业技术体系专项资金(CARS-09)
Effects of selenium application on Se content, nutritional compositions and antioxidant capacity in faba bean seeds
ZHANG Hua-hua1, LI Fang1, LI Hang-yu1, GE Jun-yong1, XU Dong-xu2, KANG Yu-fan1
(1.College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China;2.Zhangjiakou Academy of Agricultural Sciences, Zhangjiakou 075000, China)
Abstract:
Selenium (Se) is an essential micronutrient for both human and animals, and also affects plant metabolism.In this study, the effects of increasing doses of Se application (0, 3.1, 6.2, 12.4, 24.8 mg Se/kg) on the yield, Se content, nutritional and anti-nutritional substances as well as antioxidant capacity in grains of two faba bean cultivars were investigated under a pot experiment.The analysis showed that increasing Se concentrations correlated positively with Se content in grains and negatively with yield and raw protein content.Se contents of Chongli and Fengdou No.6 increased by 21.3 to 35.8 times and 4.97 to 7.60 times compared with each control, respectively.In addition, Se application enhanced tannin content and 1, 1-Diphenyl-2-picrylhydrazyl radical 2, 2-Diphenyl-1-(2, 4, 6-trinitrophenyl) hydrazyl (DPPH) free-radical-scavenging potential.However, the contents of total carbohydrates and phytic acid were affected slightly and were not statistically significant.The results also indicated that effects of Se on those changes studied differed among faba bean varieties.Therefore, for the production of Se-enriched food the recommended Se concentrations should be based not just on Se content in grains, but also on the yield and changes of both nutrients and anti-nutrients.Se treatment at low concentration of 3.1 mg/kg was suitable for production of Se-enriched faba bean according to this study.
Key words:  selenium  faba bean  nutrient  anti-nutrient  DPPH free-radical-scavenging potential