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| 外源施加褪黑素对NaCl胁迫下狼尾草种子萌发及相关生理指标的影响 |
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张娜1, 蒋庆1, 李殿波1, 蔡林桐1, 张海军1, 司文君2, 范希峰3, 郭仰东1
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| (1.中国农业大学 农学与生物技术学院/国家能源非粮生物质原料研发中心, 北京 100193;2.北京市昌平区马池口镇农业服务中心, 北京 102200;3.北京市农林科学院 草业与环境研究中心, 北京 100097) |
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| 摘要: |
| 在狼尾草种子萌发过程中外源施加褪黑素，观察测定NaCl胁迫下狼尾草种子的萌发特性和萌动种子体内的抗逆酶活性与相关生理生化指标。结果表明：褪黑素处理显著提高了NaCl胁迫下狼尾草种子的发芽势和发芽率，其中300和500 μmol/L的褪黑素处理效果比较明显。褪黑素处理显著提高了狼尾草胚根的直径，起到了壮根的作用。NaCl胁迫下褪黑素处理组的平均干重显著高于非褪黑素处理的组别，表明褪黑素处理促进了干物质积累。另外褪黑素提高了种子体内的游离脯氨酸和可溶性蛋白含量，有助于抵抗盐胁迫逆境造成的渗透伤害；提高了抗氧化系统酶的活性（SOD、POD和CAT），降低了丙二醛的含量，有效防止了胁迫产生的多余活性氧所造成的过氧化伤害。综合以上数据，褪黑素具有提高狼尾草抵抗NaCl胁迫逆境的作用。 |
| 关键词: 狼尾草 褪黑素 NaCl胁迫 发芽率 抗氧化 |
| DOI：10.11841/j.issn.1007-4333.2014.04.08 |
| 投稿时间：2013-12-25 |
| 基金项目:国家“863”计划项目（2012AA101801）；“十二五”国家科技支撑计划项目（2011BAD17B01） |
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| Effect of exogenous melatonin on germination of Pennisetum alopecuroides under NaCl stress |
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ZHANG Na1, JIANG Qing1, LI Dian-bo1, CAI Lin-tong1, ZHANG Hai-jun1, SI Wen-jun2, FAN Xi-feng3, GUO Yang-dong1
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| (1.College of Agromomy and Biotechnology/National Energy R & D Center for Non-food Biomass, China Agricultural University, Beijing 100193, China;2.Ma Chi Kou Agricultural Service Center, Changping District, Beijing 102200, China;3.Beijing Research and Development Center for Grass and Environment, Beijing Academy of Agricultural and Forestry Sciences, Beijing 100097, China) |
| Abstract: |
| In this study,we measrued the germination characteristics,anzyme activities and related physiological characteristics of pennisetum treated with exogenous melatonin under NaCl stress.Melatonin treatment improved the germination rate at 48 h and the final germination rate.The M300 and M500 treatments had high effect.Melatonin enhanced the root system of pennisetum by increasing the radical diameter.The melatonin treated group had larger dry weight than that of control group,indicating that melatonin facilitated the dry matter accumulation.In addition,both free proline accumulation and soluble protein content were also increased by exogenous melatonin.Both proline and soluble protein are assumed to resist the permeability damage caused by salt stress.Exogenous melatonin may keep the pennisetum from oxidative damage caused by reactive oxygen species,as improving the activities of antioxidate system enzymes (SOD,POD and CAT) and maitaining the MDA content at a low level.In summary,melatonin may be the reason for the pennisetum to resist NaCl stress. |
| Key words: Pennisetum alopecuroides melatonin NaCl stress germination antioxidation |
