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| 陆地棉蛋白质二硫键异构酶基因（GhPDIL5-2a）的克隆及其在花药中的表达分析 |
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刘冬梅1,2, 金刚3, 孔祥军1, 周步进1, 周瑞阳1
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| (1.广西大学 农学院, 南宁 530005;2.商丘师范学院, 河南 商丘 476000;3.广西壮族自治区亚热带作物研究所, 南宁 530001) |
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| 摘要: |
| 利用电子克隆的方法设计特异引物，克隆陆地棉的1个二硫键异构酶（Protein disulphide isomerase like）基因的全长序列。序列分析表明：该基因没有内含子，开放阅读框的长度为1 293 bp，编码的多肽序列含有430个氨基酸。该基因与红麻HcPDIL5-2a基因具有97%的相似性，将该基因命名为棉花GhPDIL5-2a。取单核期花药进行半定量RT-PCR分析发现：GhPDIL5-2a在陆地棉洞A不育株和可育株花药中表达无差异，在供试的其他不同品种中的表达也无差异。 |
| 关键词: 陆地棉 PDIL 克隆 表达 |
| DOI：10.11841/j.issn.1007-4333.2014.04.03 |
| 投稿时间：2013-10-27 |
| 基金项目:国家自然科学基金资助项目（31360348） |
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| Molecular cloning and expression analysis of a protein disulphide isomerase gene in upland cotton |
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LIU Dong-mei1,2, JIN Gang3, KONG Xiang-jun1, ZHOU Bu-jin1, ZHOU Rui-yang1
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| (1.College of Agriculture, Guangxi University, Nanning 530005, China;2.Shangqiu Normal University, Shangqiu 476000, China;3.Guangxi Subtropical Crops Research Institute, Nanning 530005, China) |
| Abstract: |
| The full length of a gene for protein disulphide isomerase (PDIL) in cotton was cloned through e-clone.Sequence analysis showed that introns were absent in the gene.It contained a 1 293 bp open reading frame,and encoded a deduced protein of 430 amino acid residues,and showed 97% homology to HcPDIL5-2a which regulated fertility in kenaf.This gene was tentatively designated as GhPDIL5-2a,and had the same expression pattern in DONG A sterility line and fertile line,and also no differences in other varieties. |
| Key words: upland cotton(Gossypium hirsutum L.) PDIL gene clone expression |
