| 摘要: |
| 为探讨DREBs在果实发育及成熟调控中的作用及机理，以番茄‘Micro-Tom’为试材，克隆得到1个DREBs转录因子SlDREB1B。研究证实，在果实中SlDREB1B的表达可受脱水胁迫和温度胁迫诱导。将SlDREB1B构建到带有e-GFP报告基因的载体上，采用叶盘法将SlDREB1B基因转入番茄，经报告基因筛选和SlDREB1B表达检测，成功获得转基因阳性植株。和转空载基因的植株相比，转SlDREB1B植株的果实发育和成熟进程明显加速。进一步分析表明，SlDREB1B的过量表达，可调控一系列与果实发育和成熟相关的基因表达，其中，色素合成相关基因和果实软化相关基因的表达受到强烈诱导，说明SlDREB1B可通过调节果实成熟相关基因的表达影响番茄果实发育和成熟进程。 |
| 关键词: 番茄 Micro-Tom SlDREB1B 转基因 果实 |
| DOI：10.11841/j.issn.1007-4333.2014.03.10 |
| 投稿时间：2014-02-19 |
| 基金项目:国家自然科学基金资助项目（31171921） |
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| Over-expression of SlDREB1B promotes fruit development and ripening in‘Micro-Tom’plant |
|
LI Chen, FAN Yi-juan, JI Wen-long, WEI Ling-zhi, JIANG Jin-zhu, LI Bing-bing, JIA Wen-suo
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| (College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China) |
| Abstract: |
| This study aims to understand the roles of DREBs in fruit development and ripening.With‘Micro-Tom’as research materials, a member of DREBs, SlDREB1B, was cloned.It was found that SlDREB1B could be induced by dehydration and cold stress in‘Micro-Tom’fruit.SlDREB1B was constructed into e-GFP-containing vector and transformed into‘Micro-Tom’.SlDREB1B transgenic plants were successfully obtained by e-GFP screening and SlDREB1B expressional analysis.Compared with control plants carrying empty vector, over-expression of SlDREB1B significantly promoted fruit ripening.Further analysis showed that over-expression of SlDREB1B resulted in a significant increase in many ripening related genes, especially for pigment metabolic genes and fruit softening related genes. |
| Key words: tomato Micro-Tom SlDREB1B gene transgene fruit |
